Date published: 2025-9-9

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GAIP 抗体 (A-10): sc-365157

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说明书
  • GAIP抗体(A-10)是小鼠单克隆IgG1 (kappa轻链),规格为200 µg/ml
  • 特异性抗原位于human物种的GAIP的C-terminus的氨基酸189-216之间
  • 推荐用于 mouse, rat 和 human 来源的GAIP WB, IP, IF 和 ELISA检测
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRPm-IgGκ BP-HRP是GAIP Antibody (A-10) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与GAIP Antibody (A-10) 打包提供(请参阅下面的订购信息)。

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    异源三聚体G蛋白的作用是将信息从细胞表面受体传递到细胞内效应器。在哺乳动物中,G蛋白α、β和γ多肽分别由至少16、4和7个基因编码(2-6)。对G蛋白的研究主要集中在其α亚基上,因为这些蛋白结合并水解GTP,并最明显地调节研究最多的效应器的活性。已经鉴定出四种Gα GTP酶激活蛋白(GAPs),并分别命名为RGS1(G蛋白信号调节剂)、RGS4、RGS10和GAIP(Gα相互作用蛋白)。这些蛋白质中的每一种都已被证明可以通过增加它们将GTP转化为GDP的速率来使特定的Gα异构体失活。RGS1、RGS4和GAIP紧密结合并对Gαi、Gαo和Gαt表现出GAP活性,但对Gαs没有。RGS10增加了Gαi亚家族几个成员的GTP水解活性,包括Gαi-3、Gαz和Gαo。

    仅限研究使用。不适用于诊断和治疗用途。

    订购信息

    产品名称产品编号规格价格数量收藏夹

    GAIP 抗体 (A-10)

    sc-365157
    200 µg/ml
    $316.00

    GAIP (A-10): m-IgG Fc BP-HRP 套装

    sc-537781
    200 µg Ab; 10 µg BP
    $354.00

    GAIP (A-10): m-IgGκ BP-HRP 套装

    sc-535164
    200 µg Ab; 40 µg BP
    $354.00

    GAIP (A-10): m-IgG1 BP-HRP 套装

    sc-545348
    200 µg Ab; 20 µg BP
    $354.00

    GAIP (A-10) 中和勝肽

    sc-365157 P
    100 µg/0.5 ml
    $68.00

    Hi, I ordered GAIP Antibody (A-10): sc-365157 for western blot analysis. But I am wondering, if I can use 5% non fat milk in TBS-tween buffer for the dilution of this antibody. I would be happy to get your feedback. With kind regards, Rahul

    Asked by: rm0001de
    Thank you for your question. You can find our recommended protocol for western blotting at the bottom of this page under 'Resources'. We recommend using either TBS Blotto A: sc-2333 (based on milk powder) or TBS Blotto B: sc-2335 (BSA-based). Use UltraCruz® Blocking Reagent (sc-516214) for blocking Immobilon®-FL PVDF membranes.
    Answered by: Tech Support Europe
    Date published: 2019-09-19

    What application is the blocking peptide sc-365157 P appropriate for?

    Asked by: jenniferc
    Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
    Answered by: Technical Support
    Date published: 2017-02-27
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    Rated 5 out of 5 by from Produced great immunofluorescence cytoplasmicProduced great immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
    Date published: 2015-01-15
    Rated 5 out of 5 by from Satisfactory Western Blot data of GAIP expressionSatisfactory Western Blot data of GAIP expression in non-transfected and human GAIP transfected 293 whole cell lysates. -SCBT QC
    Date published: 2013-09-04
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    GAIP 抗体 (A-10) is rated 5.0 out of 5 by 2.
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