Electrophoresis Sample Buffer, 2X A ready-to-use reducing sample buffer for SDS-PAGE.

Electrophoresis Sample Buffer, 2X

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Electrophoresis Sample Buffer, 2X is rated 4.38 out of 5 by 8.
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备用名: Electrophoresis Sample Buffer, 2X is known as the most common SDS-PAGE sample buffer.
应用; Electrophoresis Sample Buffer, 2X is a ready-to-use reducing sample buffer for SDS-PAGE, acting on denatured proteins in the system.
仅供科研使用。不可用于诊断或治疗。
* 参考分析证明 大量特定数据 (包括水 含量).
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Electrophoresis Sample Buffer, 2X is a ready-to-use reducing electrophoresis sample buffer solution with bromophenol blue for the preparation of protein samples to be separated in SDS-PAGE. Bromophenol Blue is included with Electrophoresis Sample Buffer, 2X as a tracking dye to monitor the sample during the electrophoretic run. Electrophoresis Sample Buffer, 2X contains DTT (sc-29089), which disrupts disulfide bonds to ensure proteins are fully denatured before loading on the gel. Electrophoresis Sample Buffer, 2X also contains glycerol, TCEP, DTT, SDS, 1.0M Tris-HCl pH 6.7 and bromophenol blue. For non-reducing SDS-PAGE, Electrophoresis Sample Buffer 2X (non-reducing) (sc-45085) is recommended.


参考文献

1. Toyoda, E. et al. 2005. Pancreas. 30(2): 168-173. PMID: 15714139
2. Heinz, L.X. et al. 2006. Blood. 107(4): 1445-1453. PMID: 16223775
3. Gohil, K. et al. 2008. Brain Res. 1201 : 167-176. PMID: 18299118
4. Popovici, R.M. et al. 2008. Endocrinology. 149(3): 1136-1143. PMID: 18048494
5. Tang, Y. et al. 2010. J. Nutr. Biochem. 21(3): 237-246. PMID: 19369054

用法 :
Mix sample with an equal volume of Electrophoresis Sample Buffer, 2X. Bring to 95° C for 10 minutes, cool to room temperature before loading. If particulate is present, centrifuge samples 5 minutes at 14k RPM in microcentrifuge, and load the gel.
外观 :
Aqueous solution
物理状态 :
Liquid
保存 :
Store at 4° C
仅供科研使用。不可用于诊断或治疗。

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分析证明书

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PMID: # 34142127  Rios, JJ.|Denton, K.|Yu, H.|Manickam, K.|Garner, S.|Russell, J.|Ludwig, S.|Rosenfeld, JA.|Liu, P.|Munch, J.|Sucato, DJ.|Beutler, B.|Wise, CA.| et al. 2021. Dis Model Mech. 14:

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