m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred secondary detection reagent for Dysadherin Antibody (D-10) for WB applications. This reagent is now offered in a bundle with Dysadherin Antibody (D-10) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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Dysadherin Antibody (D-10) is a high quality monoclonal Dysadherin antibody (also designated Dysadherin antibody) suitable for the detection of the Dysadherin protein of mouse origin. Dysadherin Antibody (D-10) is available as the non-conjugated anti-Dysadherin antibody. Dysadherin (FXYD domain-containing ion transport regulator 5) is a cancer-associated cell membrane glycoprotein. Dysadherin down- regulates the expression of E-cadherin, the prime mediator of cell-cell adhesion in epithelial cells, by a posttranscriptional mechanism. Decreasing intercellular adhesiveness facilitates the metastasis of cancer cells. Dysadherin is present in spleen, lung, skeletal muscle, and testis tissue, and maps to human chromosome 19q13.2.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
What is the recommended fixation method for immunofluorescence staining with Dysadherin (D-10): sc-377163 monoclonal antibody?
Asked by: jenni
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: