cyclin E抗体 (HE12) 推荐用于 mouse, rat 和 human 来源的cyclin E (doublet at 50 kDa and single band at 42 kDa) WB, IP, IF 和 IHC(P)检测

cyclin E 抗体 (HE12): sc-247

cyclin E抗体 (HE12) is rated 4.7 out of 5 by 19.
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说明书

    • cyclin E抗体(HE12)是小鼠单克隆IgG1 κ, 在753篇文献中引用,规格为200 µg/ml
    • 免疫human物种的重组cyclin E
    • 抗-cyclin E 抗体 (HE12) 推荐用于 WB, IP, IF 和 IHC(P),检测mouse, rat 和human 来源的 cyclin E (doublet at 50 kDa and single band at 42 kDa); Highly expressed in testis and placenta. Low levels in bronchial epithelial cells.
    • cyclin E 抗体 (HE12) 可偶联 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 594 用于IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 关于如何获取cyclin E (HE12): sc-247免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP 是 cyclin E Antibody (HE12) for WB and IHC(P)首选应用二抗。这些试剂现在以套装形式提供cyclin E Antibody (HE12) (见下方订购信息).
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Which buffer to use for western blot? I used 5% milk in TBS-T for blocking and for both primary and secondary Ab and got no band. Thanks

Asked by: Yaelle
Thank you for your question. We recommend using 5% nonfat milk in TBST for blocking and for both primary and secondary antibody incubations. Please contact our Technical Service Department for assistance in troubleshooting this antibody.
Answered by: Technical Support
Date published: 2019-11-25

Is the antibody directed against the whole protein or any particular site (c-or n-terminal?)

Asked by: portland
Thank you for your question. This antibody was made against a recombinant synthetic peptide corresponding to Human Cyclin E1. The exact epitope has not been epitope mapped, so I do not have further information regarding any particular site. If you have any further questions, please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com.
Answered by: Tech Service
Date published: 2018-02-07

Can the antibody used on both rat and mouse source samples? How long will it take to get the antibody?

Asked by: Guanwen
Thank you for your question. cyclin E Antibody (HE12): sc-247 is recommended for detection of cyclin E in mouse, rat and human samples. Please contact our Asian Support Office to inquire about current product availability and shipping and delivery times for your institution. Please call our Asian Support office toll-free at 800-820-8626, email [email protected], or contact us by live chat.
Answered by: Technical Support
Date published: 2017-04-07

Does it recognize both CCNE1 and CCNE2? thanks!

Asked by: NickNR
Thank you for your question. cyclin E Antibody (HE12): sc-247 is recommended for detection of cyclin E (CCNE1). We have not tested the antibody for reactivity against CCNE2.
Answered by: Technical Support
Date published: 2017-03-30

Is it possible to use this mouse monoclonal antibody to detect cyclin E in canine samples?

Asked by: Trav11
To inquire about additional species reactivity not listed on our website, please contact our Technical Service department at 800-457-3801 and we will be more than happy to run sequence analysis for your desired species.
Answered by: Tech Service 8
Date published: 2016-12-16

Can I use the cyclin E Antibody (HE12) for immunofluorescence if I am working with mouse tissue samples?

Asked by: Trav11
If looking to perform immunofluorescence in mouse tissue samples, we recommend using our directly conjugated mouse monoclonal primary antibodies. Currently, the cyclin E Antibody (HE12): sc-247 is available conjugated to fluorescein (sc-247 FITC, 200 µg/ml) and TRITC (sc-247 TRITC, 200 µg/ml).
Answered by: Tech Service 8
Date published: 2016-12-16
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Rated 5 out of 5 by from western 没有申请小样,直接买的大包装抗体,用起来相当不错,K562细胞制备的蛋白样品。所以强烈推荐,毕竟性价比还是不错的!
Date published: 2020-12-20
Rated 5 out of 5 by from Good antibody for WB I used this antibody in western blot analysis obtaining a clear signal at the predicted position
Date published: 2020-09-08
Rated 5 out of 5 by from good product It is a good antibody with clean background for WB.
Date published: 2018-09-11
Rated 5 out of 5 by from Very strong signal Sharp and strong band in westen blot carried out on samples derived from human primary macrophages.
Date published: 2018-03-12
Rated 4 out of 5 by from HE12, Santa Cruz: a good antibody This antibody works really well and for this reason we choose it among all the other that we had the possibility to test.
Date published: 2018-01-18
Rated 5 out of 5 by from Good antibody! 1:200 diluted for wb of rat heart protein,the band is strong and special
Date published: 2017-12-30
Rated 5 out of 5 by from a good antibody for WB This antibody generates a clear signal at the predicted position on the WB (1:500 dilution, 40ug HeLa extract).
Date published: 2017-09-18
Rated 5 out of 5 by from Strong signal I used cyclin E (HE12) antibody (1:500) dilution to check S phase in cell cycle experiment and got a strong signal and I can see a difference among my samples.
Date published: 2017-08-25
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