cyclin B1抗体 (GNS1) 推荐用于 mouse, rat 和 human 来源的cyclin B1 WB, IP, IF 和 IHC(P)检测

cyclin B1 抗体 (GNS1): sc-245

cyclin B1抗体 (GNS1) is rated 4.6 out of 5 by 40.
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说明书

    • 抗-cyclin B1 抗体 GNS1 是小鼠单克隆 IgG1 κ,cyclin B1抗体, 在1,101篇文献中引用,规格为200 µg/ml
    • raised against a recombinant protein corresponding to human cyclin B1
    • 抗-cyclin B1 抗体 (GNS1) 推荐用于 WB, IP, IF 和 IHC(P),检测mouse, rat 和human 来源的 cyclin B1
    • 抗-cyclin B1 抗体 (GNS1) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 还可偶联TRITC 以及 Alexa Fluor® 405 用于IF, IHC(P) 和 FCM
    • 可用于ChIP的TransCruz试剂(sc-245 X, 200 µg/0.1 ml)
    • 关于如何获取cyclin B1 (GNS1): sc-245免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgG Fc BP-HRPm-IgGκ BP-HRP 是 cyclin B1 Antibody (GNS1) for WB and IHC(P) 的首选应用二抗。这些试剂现在以套装形式提供cyclin B1 Antibody (GNS1)。 (见下方订购信息).
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Will this antibody react with bovine protein? Than you.

Asked by: clvleal
Thank you for your question. We are aware of one publication citing use of this antibody in bovine ABAE cells. Please refer to this reference on NCBI's PubMed, PMID: 15746189.
Answered by: Technical Support
Date published: 2017-08-02

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: Sweetums
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 4
Date published: 2017-11-03

I need to use an antibody to detect the protein in samples of (other species, e.g., bovine, porcine, canine, equine, avian, non-mammalian) origin. Do you know if this mouse monoclonal antibody will work?

Asked by: Sweetums
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Tech Service 4
Date published: 2016-12-16

Hi, do you already know if this antibody will react with bovine cells/tissue?

Asked by: clvleal
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Rated 4 out of 5 by from Good for western blot The antibody can detect a specific band with 1:250 dilution for western blot. Samples were HeLa cell extract.
Date published: 2019-10-08
Rated 5 out of 5 by from anti-cyclin B1 antibody works for western blot. We used the anti-cyclin B1 antibody for western blotting of HeLa whole cell lysate and detected a band of the correct size.
Date published: 2019-07-29
Rated 1 out of 5 by from No Western signal in INS-1 rat cell lysates Tested by Western Blot (Li-Cor reagents) of rat insulinoma (INS-1) cell line. 60 ug loads were probed overnight, but no bands were detected. Alternatively, faint bands detected using sc-7393
Date published: 2019-05-22
Rated 5 out of 5 by from Figure 5-D Protein analysis by Western blot analysis of CCNB1 in three mutant lung adenocarcinoma cells (H1792.604, H2009, H358)
Date published: 2019-02-22
Rated 5 out of 5 by from wonderful antibody! 1:200 diluted for wb of rat protein ,the band is strong and special
Date published: 2018-06-12
Rated 5 out of 5 by from Good for WB This antibody is good for Western blot using human cell nuclear extract.
Date published: 2018-05-12
Rated 5 out of 5 by from Great product!! It's a great product for me to analyze Western blotting.
Date published: 2018-04-22
Rated 5 out of 5 by from 使用效果还不错 近期刚使用这个抗体,按照1:400的浓度测试,发出的条带符合说明书的介绍。清晰度之类符合预期,比较满意。
Date published: 2018-04-16
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