cyclin B1抗体 (0.N.238) 推荐用于 mouse, rat 和 human 来源的cyclin B1 WB, IP, IF, IHC(P) 和 KA检测

cyclin B1 抗体 (0.N.238): sc-70898

cyclin B1抗体 (0.N.238) is rated 4.5 out of 5 by 2.
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说明书

    • cyclin B1抗体(0.N.238)是小鼠单克隆IgG1 κ, 在15篇文献中引用,规格为200 µg/ml
    • raised against a recombinant protein corresponding to human cyclin B1
    • 推荐用于 mouse, rat 和 human 来源的cyclin B1 WB, IP, IF, IHC(P) 和 KA检测
    • 看 cyclin B1 (GNS1): sc-245,可查看有标记的 cyclin B1 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
    • 对于cyclin B1 抗体 (0.N.238)的WB and IHC(P)应用,m-IgGκ BP-HRP 是首选的二抗检测试剂。现在该试剂和cyclin B1 抗体 (0.N.238)组合成套装提供(参见下面的订购信息)。对于其他m-IgGκ BP 偶联产品,参见我们小鼠IgG 结合蛋白的完整列表
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How much sample do you recommend loading on the gel for Western blot detection using cyclin B1 (0.N.238): sc-70898?

Asked by: TinTin
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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Rated 4 out of 5 by from Band at expected size with transfected lysate Band at expected size with transfected lysate and K-562 nuclear extract -SCBT QC
Date published: 2015-01-30
Rated 5 out of 5 by from Published data in IF application with U2OS Published data in IF application with U2OS cell lines with and without the constitutive expression of Epstein-Barr virus (EBV) EBNA1 protein and polyomavirus large T antigen -SCBT Publication Review
Date published: 2013-01-22
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