COL7A1抗体 (4D2) 推荐用于 human 来源的Collagen Type VII WB, IP, IF 和 IHC(P)检测

COL7A1 抗体 (4D2): sc-33710

COL7A1抗体 (4D2) is rated 5.0 out of 5 by 4.
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说明书

    • 抗-COL7A1 抗体 4D2 是小鼠单克隆 IgG1 κ,COL7A1抗体, 在19篇文献中引用,规格为200 µg/ml
    • raised against SDS-insoluble proteins of normal human skin
    • COL7A1 抗体 (4D2) 推荐用于WB, IP, IF 和 IHC(P),检测human 来源的 Collagen Type VII
    • 抗-COL7A1 抗体 (4D2) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 关于如何获取COL7A1 (4D2): sc-33710免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgG Fc BP-HRP (小鼠IgG Fc 结合蛋白偶联辣根过氧化物酶(HRP)) and m-IgG1 BP-HRP (mouse IgG1 binding protein-HRP) 是 COL7A1 Antibody (4D2) for WB and IHC(P)首选应用二抗。这些试剂现在以套装形式提供COL7A1 Antibody (4D2) (见下方订购信息).

Do you know if this antibody (COL7A1 Antibody (4D2): sc-33710) reacts with mouse, or only human? We are searching for an anti-COL7A1 antibody that will NOT recognized mouse collagen VII, only human, and is conjugated. Thanks for the help.

Asked by: Dorothy
Thank you for your question. We recommend sc-33710:COL7A1 (4D2) for use with human samples. The exact binding site of sc-33710:COL7A1 (4D2) has not been characterized and, therefore, unable to determine homolgy with mouse COL7A1. We offer sc-33710:COL7A1 (4D2) conjugated to HRP and a variety of fluorochromes. If you are interested, we are providing complimentary samples of the unconjugated version of this antibody for a limited time. Please contact technical service for further information.
Answered by: Santa Cruz TS
Date published: 2017-05-19

Can COL7A1 (4D2): sc-33710 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Randy McDonald
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, COL7A1 (4D2): sc-33710 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Strong IF staining We used this antibody for IF staining and were able to produce very sharp images showing membrane localization
Date published: 2017-01-25
Rated 5 out of 5 by from IHC on paraffin embedded sections of human IHC on paraffin embedded sections of human skin (dilution 1/50) - Published data
Date published: 2015-08-07
Rated 5 out of 5 by from Antibody works great for IHC paraffin Antibody works great for IHC paraffin-embedded human kidney tissue. -SCBT QC
Date published: 2015-03-20
Rated 5 out of 5 by from Worked well for immunofluorescence staining Worked well for immunofluorescence staining of normal human cornea frozen section. -SCBT QC
Date published: 2015-01-11
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