COL6A1抗体 (B-4) 推荐用于 mouse, rat 和 human 来源的Collagen α1 Type VI WB, IP, IF 和 ELISA检测

COL6A1 抗体 (B-4): sc-377143

COL6A1抗体 (B-4) is rated 4.6 out of 5 by 10.
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说明书

    • 抗-COL6A1 抗体 B-4 是小鼠单克隆 IgG2a κ,COL6A1抗体, 在6篇文献中引用,规格为200 µg/ml
    • 特异性抗原位于mouse物种的Collagen α1 Type VI的内部区域内的氨基酸53-87之间
    • 抗-COL6A1 抗体 (B-4) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 Collagen α1 Type VI
    • 抗-COL6A1 抗体 (B-4) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 关于如何获取COL6A1 (B-4): sc-377143免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。

Has the antibody been used for flow cytometry?

Asked by: Noor
Thank you for your question. This antibody has not yet been tested for Flow cytometry and so that is not a recommended application at this time. We are still gathering data to show on our website. Check back for updates!
Answered by: Tech Service
Date published: 2019-11-15

Hi, do you have any data that this antibody works for immunoprecipitation? Yes, I see that in the description you claim it does. But I'm wondering if the Western blot images came from post-IP. Thank you, David

Asked by: David K
Thank you for your question. The Western blot data shown on our website currently is from a direct Western blot, without any immunoprecipitation of the tested whole cell lysates. This primary antibody is recommended and warrantied for immunoprecipitation (IP), however we are still gathering data to show on our website. Check back for updates!
Answered by: Technical Support
Date published: 2019-04-04

What application is the blocking peptide sc-377143 P appropriate for?

Asked by: jenni
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from worked welll for WB of mouse tissue extracts Worked well with standard WB conditions: 20-60μg protein (total extracts in RIPA buffer) per sample was loaded. Membranes were blocked with 5% BSA or non-fat dry milk in PBS-T(1% Tween 20). Antibody was diluted 1/500.Blots were incubated with the appropriate secondary horseradish peroxidase-coupled antibody (GE) and developed using the Luminata Western HRP Substrate (Millipore) and Amersham ECL Hyperfilms (GE Healthcare) or a ChemiDoc XRS+ imaging system with Image Lab image acquisition and analysis software (Bio-Rad)
Date published: 2019-11-28
Rated 5 out of 5 by from Works for WB Clear band of correct size by WB (human breast cancer cell lysates).
Date published: 2019-04-19
Rated 2 out of 5 by from Tested on HFFF by FACS This antibody was tested on HFFF (human) cells by FACS and did not give a signal , it therefore does not react with human col6A1
Date published: 2018-11-19
Rated 5 out of 5 by from Excellent antibody for WB I got this antibody sample months ago. It work very good for me in WB of adipose tissue.
Date published: 2017-10-20
Rated 5 out of 5 by from works good in IHC i received a sample for this antibody and tried it on human skin FFPE sections. It stains very well.
Date published: 2017-07-05
Rated 5 out of 5 by from excellent antibodies We purchased many antibodies and reagents from Santa Cruz. Those are very good quality!
Date published: 2017-05-22
Rated 5 out of 5 by from Great antibody for WB Used for western blot human lungs tissue,the good result made my experiment very successful.at 1:500 dilution.
Date published: 2017-01-26
Rated 5 out of 5 by from Works well for IF with HeLa samples Works well for IF with HeLa samples. -SCBT QC
Date published: 2015-03-25
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