COL5A1抗体 (C-5) 推荐用于 mouse, rat 和 human 来源的Collagen α1 Type V WB, IP, IF, IHC(P) 和 ELISA检测

COL5A1 抗体 (C-5): sc-166155

COL5A1抗体 (C-5) is rated 4.4 out of 5 by 7.
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说明书

    • 抗-COL5A1 抗体 C-5 是小鼠单克隆 IgG2a κ,COL5A1抗体, 在5篇文献中引用,规格为200 µg/ml
    • 免疫human来源Collagen α1 Type V位于胞外域的251-450氨基酸
    • 抗-COL5A1 抗体 (C-5) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 Collagen α1 Type V
    • 抗-COL5A1 抗体 (C-5) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 关于如何获取COL5A1 (C-5): sc-166155免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgG Fc BP-HRP (小鼠IgG Fc 结合蛋白偶联辣根过氧化物酶(HRP))m-IgGκ BP-HRP (小鼠IgGκ 结合蛋白偶联辣根过氧化物酶(HRP)) 是 COL5A1 Antibody (C-5) for WB and IHC(P) 的首选应用二抗。这些试剂现在以套装形式提供COL5A1 Antibody (C-5)。 (见下方订购信息).

I am using COL5A1 (C-5): sc-166155 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Cweed
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 3 out of 5 by from 蛍光免疫染色 4%PFA固定したラット組織の凍結切片と、賦活化処理したパラフィン切片に対して蛍光免疫染色を行いましたが、凍結切片では特異的なシグナルが得られましたが、パラフィン切片ではシグナルは見られません。
Date published: 2018-11-26
Rated 4 out of 5 by from Detected a weak but specific band in WB This antibody is good for COL5a1 in WB. Specific band observed in WB of adipose tissue of mouse.
Date published: 2017-10-20
Rated 5 out of 5 by from works great on western blot and IHC We have used this antibody on human skin tissue. It works great on both western blot and IHC. It stains endothelial cells, fibroblasts and pericytes very well, but not in dermal matrix.
Date published: 2017-09-20
Rated 5 out of 5 by from Well-rounded antibody for COL5A1 great choice if you need a strong and specific antibody for COL5A1, great results in IHC, IF and WB.
Date published: 2017-02-07
Rated 5 out of 5 by from Great staining with paraffin Great staining with paraffin-embedded human pancreas tissue. -SCBT QC
Date published: 2015-04-13
Rated 5 out of 5 by from IF: Very good staining in methanol fixed IF: Very good staining in methanol fixed HeLa cells. -SCBT QC
Date published: 2015-04-02
Rated 4 out of 5 by from Decent WB data in CCD Decent WB data in CCD-1064Sk and Hs68 whole cell lysates. -SCBT QC
Date published: 2015-03-09
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