COL3A1抗体 (B-10) 推荐用于 mouse, rat 和 human 来源的Collagen α1 Type III WB, IP, IF 和 ELISA检测

COL3A1 抗体 (B-10): sc-271249

COL3A1抗体 (B-10) is rated 4.6 out of 5 by 16.
  • y_2021, m_4, d_10, h_16
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.14
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_16
  • loc_zh_CN, sid_271249, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 113ms
  • REVIEWS, PRODUCT
5
13
4
1
3
1
2
0
1
1

说明书

    • 抗-COL3A1 抗体 B-10 是小鼠单克隆 IgG1 κ,COL3A1抗体, 在41篇文献中引用,规格为200 µg/ml
    • 免疫原氨基酸序列901-1200位于human物种的Collagen α1 Type III的C-terminus附近
    • 抗-COL3A1 抗体 (B-10) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 Collagen α1 Type III
    • 抗-COL3A1 抗体 (B-10) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 关于如何获取COL3A1 (B-10): sc-271249免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred secondary detection reagent for COL3A1 Antibody (B-10) for WB applications. This reagent is now offered in a bundle with COL3A1 Antibody (B-10) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

What is the dilution used for immunohistochemistry? Thank you

Asked by: marcelnani
Thank you for your question. We recommend a starting dilution of 1:50 for IHC applications with this antibody.
Answered by: Tech Service
Date published: 2019-12-03

Does your antibody recognize nature or denature form of human collagen III, under reducing or nonreducing condition?

Asked by: SU33
Thank you for your question. All of the published Western blot data is using this antibody under reducing and denaturing conditions. We have not tested this antibody under non-reducing conditions.
Answered by: Technical Support
Date published: 2019-04-13

I tested the COL3A1 Antibody (B-10): sc-271249 in human fibroblasts. I got a clear band but it was close to 250kD. And I found some other companies' COL3A1 antibodies also recognize a 250kD band. Does anyone have the observation?

Asked by: Zhijie
Thank you for your question. We have representative Western blot data using this specific antibody published on our website. We would recommend searching the primary literature for further information about higher molecular weight forms of COL3A1 that might be observed in Western blot.
Answered by: Technical Support
Date published: 2018-11-19

I am studying COL3A1, but not in human samples. Will this antibody work in other species?

Asked by: cjMara
COL3A1 (B-10): sc-271249 monoclonal antibody is recommended for detection of Collagen α1 Type III of mouse, rat and human origin. Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support
Date published: 2017-01-30
  • y_2021, m_4, d_10, h_16CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.14
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_4
  • loc_zh_CN, sid_271249, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 106ms
  • QUESTIONS, PRODUCT
Rated 3 out of 5 by from 条带显示不好 WB试验,上样量40ug,多次试验结果,没有明显条带出现,不知道小样的品质与正品有没有差别,看图还是有点伤心的
Date published: 2018-10-11
Rated 5 out of 5 by from Strong bands in western blot Strong bands on human dermal fibroblast cell lysate. Primary was incubated at 1:200 for 1 hour at RT. 5% milk was used as a block. Proteins were transferred on nitrocellulose.
Date published: 2018-04-02
Rated 4 out of 5 by from Col3a1 Band at the expected size >100kDa but also a smaller one around 30kDa.
Date published: 2017-12-21
Rated 5 out of 5 by from great for cardio fibroblasts It is great for WB and staining on cardio fibroblasts.
Date published: 2017-12-14
Rated 5 out of 5 by from Works really well! We used it 1:1000 diluted in 1% milk T-TBS in a western blot (9% Laemmli) for heart lissates. The bands were strong and clear and the molecular weight was about 100kDa. The antibody resulted specific and worked really well.
Date published: 2017-10-26
Rated 5 out of 5 by from Excellent We have used this antibody on human skin tissue. It works great on both western blot and IHC. It stains papillae matrix in upper dermis very well.
Date published: 2017-09-20
Rated 5 out of 5 by from good antibody This antibody is so good for western blot, it shows a clear band
Date published: 2017-07-25
Rated 5 out of 5 by from COL3A1 (B-10) antibody I have done the Western blot, the band is clear and good !
Date published: 2017-05-08
  • y_2021, m_4, d_10, h_16
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.14
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_16
  • loc_zh_CN, sid_271249, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 7ms
  • REVIEWS, PRODUCT

圣克鲁斯生物技术在生物医药研究市场的产品开发方面处于世界领先地位。欢迎 致电我们的免费电话(021) 800-820-8626。 版权 ©, 2007-2021圣克鲁斯生物技术。版权所有。“Santa Cruz Biotechnology”,圣克鲁斯生物技 术的 商标,“Santa Cruz Animal Health”,“San Juan Ranch”,“Supplement of Champions”,San Juan Ranch的商标,“Ultracruz”,“Chemcruz”,“Immunocruz”,“Exactacruz”和“EZ Touch”都是圣克鲁斯生物技术的注册商标。所有的商标都归其各自拥有者所有。