COL2A1抗体 (M2139) 推荐用于 mouse, rat, human 和 Arabadopsis 来源的Collagen Type II WB, IP, IF 和 IHC(P)检测

COL2A1 抗体 (M2139): sc-52658

COL2A1抗体 (M2139) is rated 4.8 out of 5 by 13.
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说明书

    • 抗-COL2A1 抗体 M2139 是小鼠单克隆 IgG2b κ,COL2A1抗体, 在97篇文献中引用,规格为100 µg/ml
    • raised against the collagen II J1 epitope (triple helical position 551-564) of mouse origin
    • 推荐用于 mouse, rat, human 和 Arabadopsis 来源的Collagen Type II WB, IP, IF 和 IHC(P)检测; 也和以下物种反应,包括: bovine
    • 关于如何获取COL2A1 (M2139): sc-52658免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。

I would like to detection of collagen type 2 in western blot assay. I tried dilution 1:500 in 5% skinmilk. and 2nd antibody used sc-2005 product. but It does not detection. How can I do? my sample orign is human pellet.

Asked by: vtml
Thank you for your question. We recommend starting with a 1:100 primary antibody dilution for Western blot. For further assistance, please contact Technical Service.
Answered by: Technical Support
Date published: 2019-01-18

Is trial pack available for this antibody ?

Asked by: Ghosh
Thank you for the question. COL2A1 Antibody (M2139) is a mouse monoclonal mouse IgG2b κ (kappa light chain) provided at 100 µg/ml. We currently do offer Sample sizes for this product. Please request this with your supplier. However, you can also purchase the product with our 1 year full product warranty if you are planning to use this product in any of the warranted applications and warranted species: recommended for detection of Collagen Type II of mouse, rat, human and a origin by WB, IP, IF and IHC(P); also reactive with additional species, including bovine.
Answered by: Technical Support Europe
Date published: 2017-12-03

I would like to do some IF on my mouse samples. Do you have any suggestions on how this can be successful using a mouse monoclonal primary?

Asked by: Cweed
We recommend using a a directly conjugated primary antibodies. Please contact our special orders department for pricing and availability of sc-52658 conjugates.
Answered by: Technical Support 10
Date published: 2017-01-12

I can find the donkey anti-mouse secondary that I am accustomed to using. What do you recommend as a replacement?

Asked by: Cweed
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: TechService7
Date published: 2016-12-30
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Rated 4 out of 5 by from buono in immuno e wb Ho utilizzato questo anticorpo sia in immunofluorescenza (diluizione usata 1:100), che in wb (usato 1:1000) su midollo spinale di topo e sono rimasta molto soddisfatta del risultato ottenuto
Date published: 2020-10-06
Rated 4 out of 5 by from 凍結切片で免疫染色可能 無料サンプルをご提供いただき、凍結切片の免疫染色に用いました。条件検討の結果、染色像が得られました。
Date published: 2020-09-16
Rated 5 out of 5 by from Good antibody for ICC Good antibody for ICC! Application: ICC Sample: rat chondrocytes
Date published: 2020-08-25
Rated 5 out of 5 by from Excellent for IF It works very well in whole mount immunofluorescence
Date published: 2018-04-17
Rated 4 out of 5 by from works great on western blot We have used this antibody on human skin tissue. It works great on western blot.
Date published: 2017-09-20
Rated 5 out of 5 by from COL2A1 IHC chick embryo, PFA fixed, cryosectioned 2nd Ab Alexa fluor488
Date published: 2017-09-05
Rated 5 out of 5 by from good The antibody is suitable for cellular immunofluorescence. I used it in chondrocytes.
Date published: 2017-07-03
Rated 5 out of 5 by from Well done! We used the COL2A1 (M2139) is a kind of rat monoclonal antibody IgG2b ( 100 ug/ml). Conclusion: can improve the j - 1 epitope collagen II (551-564), triple helix position of rat.
Date published: 2017-02-10
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