COL1A1抗体 (3G3) 推荐用于 mouse, rat 和 human 来源的Collagen α1 Type I WB, IP, IF, IHC(P) 和 ELISA检测

COL1A1 抗体 (3G3): sc-293182

COL1A1抗体 (3G3) is rated 4.2 out of 5 by 25.
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说明书

    • 抗-COL1A1 抗体 3G3 是小鼠单克隆 IgG3 κ,COL1A1抗体, 在79篇文献中引用,规格为100 µg/ml
    • raised against a recombinant protein corresponding to amino acids 1021-1108 of COL1A1 of human origin
    • 推荐用于 mouse, rat 和 human 来源的Collagen α1 Type I WB, IP, IF, IHC(P) 和 ELISA检测
    • 关于如何获取COL1A1 (3G3): sc-293182免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。

Would this antibody work on COL1 of porcine origin?

Asked by: KaraThrace
Thank you for your question. This antibody has not yet been tested in porcine and so that is not a recommended species at this time. We are continuously gathering data to show on our website. Check back for potential updates! If you have any further questions, please contact Technical Service.
Answered by: Technical Service 3
Date published: 2020-11-12

Would this ABY work for western analysis of the purified rat-tail collagen used for cell culture?

Asked by: Monger
Thank you for your question. We haven't tested this antibody using purified rat-tail collagen used for cell culture, so we do not know if it would work on that protein.
Answered by: Technical Support
Date published: 2020-07-24

I checked col1a1 in mice kidney extract through WB.While Band positions were detected from 52 kDa to 76 kDa, not in the same location as the protocol. Even if it is a decomposed product, the molecular weight is small. Please tell me What is it.

Asked by: MHTK
Thank you for your question. Please contact our Technical Service Department for assistance in troubleshooting this antibody.
Answered by: Technical Support
Date published: 2020-07-23

hello. I have an one question about this antibody. Can this antibody even recognize pro collagen type 1? I'm wondering if this ab can distinguish collagen fiber and pro collagen.

Asked by: finalb2
Thank you for your question. Based on the epitope mapping, this antibody should be able to recognize both pro collagen alpha1 type I, and the mature protein. We have not tested the antibody to determine if it can distinguish between collagen fiber and pro collagen, but we would recommend you review some of the product citations using this antibody for more information about how the antibody is being used.
Answered by: Technical Support
Date published: 2020-03-31

I used this antibody to check col1a1 in mice kidney extract through WB.While the reasult showed a lot of non-specific bands and i couldn't comfirm which is the col1a1. 1st dil. 1:1000. 2nd anti-mouse lgG dil.1:5000. Could u provide a positive con?

Asked by: hhx112
Thank you for your question. Please contact your local distributor for assistance in troubleshooting this antibody. They will be happy to help you!
Answered by: Tech Service
Date published: 2021-03-19

Does this antibody detect human collagen I in denaturing and/or reducing western blot?

Asked by: Whitecat88
Thanks for your question. COL1A1 (3G3) is recommended for detection of Collagen α1 Type I of mouse, rat and human origin by denaturing and/or reducing WB. If you have any further questions, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: [email protected] or by live chat directly on our website, www.scbt.com
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

Could be used to detect COL1A1 from equine MSC?

Asked by: peraltaoscar4
Thank you for your question. We have no information regarding possible reactivity of this antibody with equine COL1A1. If you are able to provide a published protein sequence for COL1A1 in equine then please contact our Technical Service Department and we can help you perform sequence analysis comparing the antibody's antigen to the equine protein sequence. Please call us toll-free at (800) 457-3801 option 2, e-mail <[email protected]>, or use the live chat on our website.
Answered by: Technical Support
Date published: 2018-03-19

What is the recommended secondary for this mouse monoclonal primary antibody?

Asked by: AbPolly
Thank you for your question. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 9
Date published: 2017-01-13
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Rated 4 out of 5 by from Bene in wb Ho provato questo anticorpo in wb (1:1000) su spinal cord di topo ed ha funzionato molto bene! Ottimo anticorpo
Date published: 2020-10-05
Rated 5 out of 5 by from Good for WB It's useful for detecting both Pro-collagen I and matured collagen I.
Date published: 2020-09-04
Rated 5 out of 5 by from good for immunohistochemistry Immunohistochemistry: Paraffin kidney section, immune infiltrate Antibody dilution: 1:50 Antigen retrieval in citrate buffer pH6 Comment: Good signal
Date published: 2020-08-10
Rated 1 out of 5 by from No encontramos señal. WB muy sucio; imposible detectar señal de ñaproteina.
Date published: 2020-05-09
Rated 4 out of 5 by from Works well in WB This antibody works well in WB, I used it to detect ColI in Huh7 cells.
Date published: 2020-02-21
Rated 3 out of 5 by from Tested on HFFF by FACS This antibody was tested on HFFF cells by FACS and gave a satisfactory signal at dilution 1:25
Date published: 2018-11-19
Rated 2 out of 5 by from 条带并不美观 买了elastin正品申请了COL1A饿小样,跑过4次,稀释浓度为1:200,跑出来的结果都不是很乐观,条带分散,拖影严重,在150.200kd附近有些许条带
Date published: 2018-10-11
Rated 5 out of 5 by from western blot antibody is good for western blot in osteoblasts , used att dilution 1
Date published: 2018-04-06
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