COL1A抗体 (COL-1) 推荐用于 mouse, rat 和 human 来源的native Collagen Type I WB, IP, IF 和 ELISA检测

COL1A 抗体 (COL-1): sc-59772

COL1A抗体 (COL-1) is rated 4.4 out of 5 by 12.
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说明书

    • 抗-COL1A 抗体 COL-1 是小鼠单克隆 IgG1COL1A抗体, 在159篇文献中引用,规格为100 µl ascites
    • raised against full length native Collagen Type I of bovine origin
    • recommended for detection of native Collagen Type I of mouse, rat and human origin by WB (non-reducing), IP, IF, IHC (frozen) and ELISA; 也和以下物种反应,包括: 和 bovine, porcine, feline and canine; 可能与 connective tissue fibers in acetone-fixed or unfixed frozen sections产生交联反应; 不会与 Collagen Types II, III, IV, V, VI, VII, IX, X and XI产生交联反应

Hi, can you suggest a protocol of western blot for detecting this native collagen from porcine samples?

Asked by: danK
Thank you for your question. You can find our recommended western blot protocol under this link: https://www.scbt.com/resources/protocols/western-immuno-blotting
Answered by: Tech Support Europe
Date published: 2021-02-03

Hello, is there an SDS document for this product? Our Health & Safety department requires one for all products we use.

Asked by: J Thorpe
Thank you for your question. We do not provide SDS documents for our antibody reagents. SDSs are only provided for chemicals. Please reach out to your local distributor, they will be able to further assist you.
Answered by: Tech Service
Date published: 2020-01-28

What is the dilution used for immunohistochemistry (P)? Thank you

Asked by: marcelnani
Thank you for your question. We recommend a starting dilution of 1:50 for staining paraffin embedded sections with this antibody.
Answered by: Tech Service
Date published: 2019-12-03

For WB, does this antibody only recognizes native proteins? No SDS and reducing reagents in loading buffer?

Asked by: qinggoushi
Thank you for your question. This antibody, COL1A, sc-59772, will only recognize the native form, so yes, do not add SDS or reducing reagents to the loading buffer.
Answered by: Tech Service
Date published: 2019-11-13

If there any recommandation of secondary antibody for IF?

Asked by: lucky7
Thank you for your question. COL1A Antibody (COL-1) is a mouse monoclonal IgG1. We recommend using as a secondary antibody for IF, our mouse IgG kappa binding protein (m-IgGκ BP) conjugated to a fluorescent probe such as fluorescein (FITC), m-IgGκ BP-FITC: sc-516140.
Answered by: Technical Support
Date published: 2019-04-13

Will this antibody stain for secreted fibrillar collagen-1? I'm unsure whether full-length native collagen type-1 means its specific for assembled collagen-1 fibrils. Will it also stain for pro-collagen chains? or only assembled fibrils? Thanks!

Asked by: Benn
Thank you for your question. This COL1A antibody recognises the native (helical) form of collagen type I.
Answered by: Tech Service
Date published: 2019-03-26

Hi, Can you supply conjugated version (either Alexa or PE) of this COL1A Antibody (COL-1): sc-59772? Thanks

Asked by: Harinath Sale
Thank you for your question. No, this antibody is provided in an ascites format and therefore is not available as a conjugated antibody. Please contact our Technical Service for recommended alternatives.
Answered by: Technical Support
Date published: 2018-12-13

Can I use this antibody for IHC-P on human skin tissue?

Asked by: moyaP
Thank you for your question. This antibody is recommended for IHC(P), or immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, and it is also suitable for detection of COL1A in human tissues. We have not yet tested the antibody in human skin tissue, however there are numerous publications citing use of this antibody for IF/IHC in human skin tissue.
Answered by: Technical Support
Date published: 2018-11-22
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Rated 1 out of 5 by from no good for MEF I bought few weeks ago. I did western blot as soon as I got it. I used this antibody by dilution 1:500. There was no specific collagen band. I only tried MEF lysate. I'm not sure this antibody works for mouse collagen. If anybody knows antibodies for mouse collagen, please give me any information. Thanks,
Date published: 2018-05-11
Rated 5 out of 5 by from good I tried this antibody in WB and got a very clear result
Date published: 2017-09-22
Rated 5 out of 5 by from for WB This COL1A antibody by dilution 1:1000, WB results are good.
Date published: 2017-05-11
Rated 5 out of 5 by from specifically stains collagen I This antibody specifically stains collagen I, which is an extra cellular matrix protein. As a result, the cell boundary of cardiomyocytes was clearly stained.
Date published: 2017-01-20
Rated 3 out of 5 by from Col 1a antibody I bought few month ago and did western blot. I got some time good signal but these day I don't detect the signal. I have no idea why signal is suddenly disappear.
Date published: 2016-12-27
Rated 5 out of 5 by from Very good native detection Best for native detection, and is specific for only COL1A
Date published: 2016-12-21
Rated 4 out of 5 by from No issues in our Western No issues in our Western. Good signal.
Date published: 2015-08-20
Rated 5 out of 5 by from Good publication data with mouse AML12 cells Good publication data with mouse AML12 cells (hepatocytes). -SCBT Publication Review
Date published: 2015-03-25
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