Clathrin Light Chain抗体 (CON.1) 推荐用于 broad species 来源的Clathrin LCA and LCB WB, IP 和 IF检测

Clathrin Light Chain 抗体 (CON.1): sc-12735

Clathrin Light Chain抗体 (CON.1) is rated 3.8 out of 5 by 8.
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说明书

    • 抗-Clathrin Light Chain 抗体 CON.1 是小鼠单克隆 IgG2b κ,Clathrin Light Chain抗体, 在13篇文献中引用,规格为200 µg/ml
    • 免疫bovine物种的clathrin light chain的氨基酸23-44
    • Clathrin Light Chain 抗体 (CON.1) 推荐用于WB, IP 和 IF,检测broad species 来源的 Clathrin LCA and LCB
    • 抗-Clathrin Light Chain 抗体 (CON.1) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 关于如何获取Clathrin Light Chain (CON.1): sc-12735免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgG2b BP-HRPm-IgGκ BP-HRP 是 Clathrin Light Chain Antibody (CON.1) for WB 首选应用二抗. 这些试剂现在以套形式提供 Clathrin Light Chain Antibody (CON.1) (见下方订购信息).
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What is the recommended fixation method for immunofluorescence staining with Clathrin LC (CON.1): sc-12735 monoclonal antibody?

Asked by: Germaine
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 4 out of 5 by from good antibody The antibody was used in triple negative breast cancer cell lines and in colon cancer cell lines at the concentration of 1:500 diluted in TBS 0,1% Tween-20 and used overnight. It produce a specific band.
Date published: 2021-10-04
Rated 4 out of 5 by from good results I got good IF images in MDCK and HeLa cells with the sample antibody from Santa except for the slightly nonselective staining in cell nuclei.
Date published: 2017-11-27
Rated 1 out of 5 by from No signal in WB Used at a 1:500 dilution on 35ug of HeLa lysate and did not get a signal after 30min exposure
Date published: 2016-12-06
Rated 4 out of 5 by from Used for studying adult stem cell/pluripotency Used for studying adult stem cell/pluripotency
Date published: 2015-04-29
Rated 4 out of 5 by from Publishable data in IF using rat PC12 cells Publishable data in IF using rat PC12 cells -SCBT Publication Review
Date published: 2015-04-26
Rated 4 out of 5 by from Publishable data in WB using cattle brain Publishable data in WB using cattle brain. -SCBT Publication Review
Date published: 2015-04-02
Rated 4 out of 5 by from Nice band seen in WB using C6 whole cell lysate Nice band seen in WB using C6 whole cell lysate. -SCBT QC
Date published: 2015-03-03
Rated 5 out of 5 by from Produced great immunofluorescence cytoplasmic Produced great immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-01-21
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