Clathrin Heavy Chain/CLTC抗体 (TD.1) 推荐用于 broad species 来源的Clathrin HC WB, IP, IF 和 IHC(P)检测

Clathrin Heavy Chain/CLTC 抗体 (TD.1): sc-12734

Clathrin Heavy Chain/CLTC抗体 (TD.1) is rated 4.5 out of 5 by 24.
  • y_2021, m_12, d_6, h_19
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.20
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_24
  • loc_zh_CN, sid_12734, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 108ms
  • REVIEWS, PRODUCT
loading
5
18
4
3
3
1
2
0
1
2

说明书

    • 抗-Clathrin Heavy Chain/CLTC 抗体 TD.1 是小鼠单克隆 IgG1 κ,Clathrin Heavy Chain/CLTC抗体, 在78篇文献中引用,规格为200 µg/ml
    • 免疫human来源Clathrin HC的N-terminus
    • Clathrin Heavy Chain/CLTC 抗体 (TD.1) 推荐用于WB, IP, IF 和 IHC(P),检测broad species 来源的 Clathrin HC
    • 抗-Clathrin Heavy Chain/CLTC 抗体 (TD.1) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 关于如何获取Clathrin Heavy Chain/CLTC (TD.1): sc-12734免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 是 Clathrin Heavy Chain/CLTC Antibody (TD.1) for WB and IHC(P) 的首选应用二抗。这些试剂现在以套装形式提供Clathrin Heavy Chain/CLTC Antibody (TD.1)。 (见下方订购信息).
loading

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with Clathrin HC Antibody (TD.1): sc-12734?

Asked by: sweetums
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 13
Date published: 2017-01-12

Can Clathrin HC Antibody (TD.1): sc-12734 monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: sweetums
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. Clathrin HC Antibody (TD.1): sc-12734 monoclonal antibody is currently available conjugated to HRP (sc-12734 HRP), phycoerythrin (sc-12734 PE), fluorescein (sc-12734 FITC), Alexa Fluor® 488 (sc-12734 AF488), and Alexa Fluor® 647 (sc-12734 AF647).
Answered by: Technical Support 13
Date published: 2017-01-12
  • y_2021, m_12, d_6, h_19CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.20
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_2
  • loc_zh_CN, sid_12734, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 115ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Excellent for immunofluorescence I tested this antibody for IF in methanol-fixed VeroE6 and Huh-7 cells. Dil: 1/200, secondary: antimouse Alexa488 (Invitrogen A21202). Excellent clathrin staining. Attached image was collected on VeroE6 cells by confocal Zeiss 800 (scale bar: 10 um).
Date published: 2020-11-26
Rated 4 out of 5 by from Work Well for IF I tried the anti Clathrin antibody for IF in FRTL-5 cells (rat cell line) and MDCK cells (dog cell line) and it worked perfectly fine!
Date published: 2018-02-23
Rated 5 out of 5 by from Works very well for western blot Confirmed strong knockdown of Clathrin by siRNA by western
Date published: 2018-02-10
Rated 4 out of 5 by from Worked for IHC I used it for IHC on mouse retina frozen section (10 um) at 1/100 dilution. I did not test it by western blot .
Date published: 2017-11-06
Rated 3 out of 5 by from Not suitable for colocalization with light chain We asked this CHC antibody from Santa, we tested it by IF in MDCK cells, it decorated the cells with clearly fluorescent spots, unfortunately, we failed to get a highly colocalized picture with CLCa (a light chain of clathrin complex)
Date published: 2017-10-07
Rated 4 out of 5 by from works great on western blot and IHC We have used this antibody on human skin tissue. It works great on both western blot and IHC.
Date published: 2017-09-20
Rated 5 out of 5 by from It works well for WB I dilute the antibody at 1:1000 and incubate at 4℃ overnight. The signal is specific.
Date published: 2017-09-18
Rated 5 out of 5 by from it worked very well It is excellent at WB, the band is clear and the protein molecular weight is in the right position.
Date published: 2017-09-12
  • y_2021, m_12, d_6, h_19
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.20
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_24
  • loc_zh_CN, sid_12734, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 9ms
  • REVIEWS, PRODUCT