CHD5抗体 (D-10) 推荐用于 mouse, rat 和 human 来源的CHD5 WB, IP, IF 和 ELISA检测

CHD5 抗体 (D-10): sc-271248

CHD5抗体 (D-10) is rated 3.6 out of 5 by 5.
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说明书

    • 抗-CHD5 抗体 D-10 是小鼠单克隆 IgG1 κ,CHD5抗体, 在3篇文献中引用,规格为200 µg/ml
    • raised against amino acids 1521-1705 mapping within an internal region of CHD5 of human origin
    • 抗-CHD5 抗体 (D-10) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 CHD5
    • 抗-CHD5 抗体 (D-10) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 可用于ChIP的TransCruz试剂(sc-271248 X, 200 µg/0.1 ml)
    • 关于如何获取CHD5 (D-10): sc-271248免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP 是 CHD5 Antibody (D-10) for WB首选应用二抗。这些试剂现在以套装形式提供CHD5 Antibody (D-10) (见下方订购信息).
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What is the recommended fixation method for immunofluorescence staining with CHD5 (D-10): sc-271248 monoclonal antibody?

Asked by: Trav11
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by from Couldn't detect it Used CHD5 at a 1:1000 dilution for WB on NuPAGE gel using HEK293T WCE. Unfortunately, we weren't able to detect anything.
Date published: 2017-09-29
Rated 4 out of 5 by from 功能还可以。 一个月前申请了试用装,用来试着做了western ,从结果来看,功能还可以。稀释浓度用了200:1。
Date published: 2017-04-24
Rated 3 out of 5 by from Seeing non specific bands in WB Seeing non specific bands in WB
Date published: 2015-05-07
Rated 5 out of 5 by from Great IF data with HepG2 samples Great IF data with HepG2 samples, strong nuclear staining. -SCBT QC
Date published: 2015-02-27
Rated 5 out of 5 by from Great for Western Great for Western, clean strong bands in mouse brain and mouse cerebellum tissue extracts. -SCBT QC
Date published: 2013-08-01
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