CENP-B抗体 (F-4) 推荐用于 mouse, rat 和 human 来源的CENP-B WB, IP, IF 和 ELISA检测

CENP-B 抗体 (F-4): sc-376283

CENP-B抗体 (F-4) is rated 4.4 out of 5 by 5.
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说明书

    • 抗-CENP-B 抗体 F-4 是小鼠单克隆 IgG1 κ,CENP-B抗体, 在4篇文献中引用,规格为200 µg/ml
    • 免疫原氨基酸序列535-599位于human物种的CENP-B的C-terminus
    • 抗-CENP-B 抗体 (F-4) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 CENP-B; 也和以下物种反应,包括: 和 equine, canine and bovine
    • 抗-CENP-B 抗体 (F-4) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 关于如何获取CENP-B (F-4): sc-376283免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgG1 BP-HRP 是CENP-B Antibody (F-4) for WB首选应用二抗. 这些试剂现在以套装形式提供CENP-B Antibody (F-4) (见下方订购信息).
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What is the recommended fixation method for immunofluorescence staining with CENP-B (F-4): sc-376283 monoclonal antibody?

Asked by: Germaine
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2018-06-26
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Rated 5 out of 5 by from Great antibody for WB! It works really well in WB. It gives a strong band at 70 kDa.Dilution 1:3000
Date published: 2017-10-19
Rated 2 out of 5 by from there is no signal in mouse oocyte I have tried this antibody in mouse MI and MII oocyte using IF at 1:50, but there was no signal detected. I had added the photo in another antibody's review.
Date published: 2017-07-24
Rated 5 out of 5 by from Immunofluorescence Staining in human cell line showed very nice results!
Date published: 2017-02-18
Rated 5 out of 5 by from Good specificity in WB in HeLa whole cell Good specificity in WB in HeLa whole cell lysate and Jurkat nuclear extract. -SCBT QC
Date published: 2015-05-07
Rated 5 out of 5 by from Robust signal in WB in human transfected Robust signal in WB in human transfected 293T whole cell lysates. -SCBT QC
Date published: 2014-12-19
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