CENP-B抗体 (C-10) 推荐用于 mouse, rat 和 human 来源的CENP-B WB, IP, IF 和 ELISA检测

CENP-B 抗体 (C-10): sc-376392

CENP-B抗体 (C-10) is rated 5.0 out of 5 by 3.
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说明书

    • CENP-B抗体(C-10)是小鼠单克隆IgG2a κ, 在2篇文献中引用,规格为200 µg/ml
    • 免疫原氨基酸序列535-599位于human物种的CENP-B的C-terminus
    • 抗-CENP-B 抗体 (C-10) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 CENP-B
    • 抗-CENP-B 抗体 (C-10) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 关于如何获取CENP-B (C-10): sc-376392免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred secondary detection reagent for CENP-B Antibody (C-10) for WB applications. This reagent is now offered in a bundle with CENP-B Antibody (C-10) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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What is the recommended fixation method for immunofluorescence staining with CENP-B (C-10): sc-376392 monoclonal antibody?

Asked by: Germaine
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Good antibody for IF The CENP-B (C-10); sc-376392 performs well for IF showing nuclear localized staining pattern.
Date published: 2017-01-19
Rated 5 out of 5 by from Produced great immunofluorescence nuclear Produced great immunofluorescence nuclear staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-04-24
Rated 5 out of 5 by from Satisfactory Western Blot data of CENP Satisfactory Western Blot data of CENP-B expression in non-transfected and human CENP-B transfected 293T whole cell lysates. -SCBT QC
Date published: 2013-12-30
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