Cdk9抗体 (D-7) 推荐用于 mouse, rat 和 human 来源的Cdk9 WB, IP, IF, IHC(P) 和 ELISA检测

Cdk9 抗体 (D-7): sc-13130

Cdk9抗体 (D-7) is rated 4.4 out of 5 by 17.
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说明书

    • 抗-Cdk9 抗体 D-7 是小鼠单克隆 IgG2b κ,Cdk9抗体, 在85篇文献中引用,规格为200 µg/ml
    • 免疫human物种的Cdk9的氨基酸204-372
    • 抗-Cdk9 抗体 (D-7) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 Cdk9
    • 抗-Cdk9 抗体 (D-7) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 另提供生物素偶联抗体,可用于WB、IHC(P)和ELISA实验
    • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-13130 X, 200 µg/0.1 ml)
    • 关于如何获取Cdk9 (D-7): sc-13130免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgG Fc BP-HRP, m-IgG2b BP-HRPm-IgGκ BP-HRP 是 Cdk9 Antibody (D-7) for WB and IHC(P) 的首选应用二抗。这些试剂现在以套装形式提供Cdk9 Antibody (D-7)。 (见下方订购信息).
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How has the specificity of this antibody to Cdk9 been validated?

Asked by: AbPolly
Thank you for your question. This specificity of this antibody has been tested by in house QC, including IF data with siRNA knockdown of Cdk9.
Answered by: Technical Support 6
Date published: 2017-01-11

Does this antibody have a blocking peptide associated with it?

Asked by: AbPolly
Thank you for your question. As this antibody was raised against a recombinant protein, unfortunately it is not possible to offer a blocking peptide for it.
Answered by: Technical Support 6
Date published: 2017-01-11
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Rated 4 out of 5 by from Great for western and IP Used at 1:500 dilution to detect endogenous protein in nuclear extract from HEK293T. 5ug antibody per 500ug nuclear extract IPed CDK9; cyclin T1 is detected with sc-271348 in CDK9 IP lane.
Date published: 2018-08-02
Rated 5 out of 5 by from Recommended for western blotting! It detected endogenous CDK9 in lysates from HEK 293T cells (1:500 dilution in 5% milk in TBS-0.1% Tween-20, overnight at 4C).
Date published: 2018-06-18
Rated 1 out of 5 by from Did not work for Chip-seq We received a sample amount of D-7 antibody which we tested in our standard chip-seq experiment pipeline. We did not have sufficient amount to test various buffers. However, the IP with D-7 antibody did not produce any peaks after sequencing.
Date published: 2018-04-10
Rated 5 out of 5 by from CDK9 antibody I bought this item last month,it works pretty well.
Date published: 2017-12-28
Rated 5 out of 5 by from Nice antibody Strong and specific band in Western blotting, publishable quality.
Date published: 2017-12-07
Rated 4 out of 5 by from Good Ab for WB It works good for western-blot in breast cancer cells.
Date published: 2017-08-09
Rated 5 out of 5 by from Good CDK9 antibody This antibody worked well for WB and IP of mouse VSMC proteins.
Date published: 2017-03-09
Rated 4 out of 5 by from Good for Western blot It works for Western blot, but not good for co-IP.
Date published: 2017-01-16
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