Cdk8抗体 (D-9) 推荐用于 mouse, rat 和 human 来源的Cdk8 WB, IP, IF, IHC(P) 和 ELISA检测

Cdk8 抗体 (D-9): sc-13155

Cdk8抗体 (D-9) is rated 4.5 out of 5 by 11.
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说明书

    • 抗-Cdk8 抗体 D-9 是小鼠单克隆 IgG1 κ,Cdk8抗体, 在16篇文献中引用,规格为200 µg/ml
    • 免疫human物种的Cdk8的氨基酸326-464
    • 抗-Cdk8 抗体 (D-9) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 Cdk8
    • 抗-Cdk8 抗体 (D-9) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 还可偶联TRITC 用于IF, IHC(P) 和FCM
    • 关于如何获取Cdk8 (D-9): sc-13155免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP 是 Cdk8 Antibody (D-9) for WB and IHC(P)首选应用二抗。这些试剂现在以套装形式提供Cdk8 Antibody (D-9) (见下方订购信息).
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Basing on the previous question and answer; So, sometimes, 77kDa is possible, right?

Asked by: yu27
Thanks for your follow-up question. Yes, it could be!
Answered by: Tech Service
Date published: 2019-11-19

The question is about molecular weight of CDK8. The first picture is about 77kDa, but the others are 55KDa, which is right? The instruction is 53kDa.

Asked by: yu27
Thank you for your question. The expected molecular weight for CDK8 is around 53-55kDa. Western blotting is a technique that separates proteins based on size - in general, the smaller the protein the faster it migrates through the gel. However, migration is also affected by other factors and so the actual band size observed may differ from that predicted. Common factors include phosphorylations, glycosylations or other post-translational modifications.
Answered by: Tech Service
Date published: 2019-11-19

Does this antibody recognise CDK19 as well?

Asked by: edimi
Thank you for your question. This antibody is specific for Cdk8, and does not cross-react with Cdk19.
Answered by: Technical Support
Date published: 2019-08-17

Can Cdk8 (D-9): sc-13155 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Cweed
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, Cdk8 (D-9): sc-13155 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by from Didnot work for WB Did not work for western blot in MCF7 breast cancer cell line
Date published: 2017-11-21
Rated 5 out of 5 by from good quality Never meet difficulties use this antibody, very easy operated at 1:500 dilution and got strong band at 53kda.I suggest the loading amount reference to the papers about the expression of the target protein.For high expresion sample loading 30ug is enough.
Date published: 2017-09-10
Rated 5 out of 5 by from VERY GOOD Antiibody I got this antibody for my research and it gives very nice band with no background. We loaded 25 ug of protein and the bands were clear and crisp.
Date published: 2017-09-04
Rated 5 out of 5 by from western blot I used this antibody for western blot and got great results.I blotted cell lysates from primary hippocampal neurons. The signal was not very powerful and no background.
Date published: 2017-08-26
Rated 4 out of 5 by from good It is strong with 1:2000 dilution but has several weak non-specific bands far away in many different types of colon cancer cells.
Date published: 2017-05-12
Rated 5 out of 5 by from good quality This antibody has good quality and specific . I found clear bands, strong signals.
Date published: 2017-05-09
Rated 5 out of 5 by from Versatile Antibody This antibody is well-cited for a variety of applications including WB, IP, IF, and even ELISA. Strong, clear signal with little to no background. Works well in a variety of species including mouse, rat, and human samples.
Date published: 2017-01-30
Rated 5 out of 5 by from Good Cdk8 control antibody for siRNA KO experiment Good Cdk8 control antibody for siRNA KO experiment -SCBT QC
Date published: 2015-04-13
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