caspase-3 p17抗体 (D-12) 推荐用于 mouse, rat 和 human 来源的caspase-3 p17 and full length procaspase-3 WB, IP, IF, IHC(P) 和 ELISA检测

caspase-3 p17 抗体 (D-12): sc-373730

caspase-3 p17抗体 (D-12) is rated 4.3 out of 5 by 30.
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说明书

    • caspase-3 p17抗体(D-12)是小鼠单克隆IgG2b κ, 在35篇文献中引用,规格为200 µg/ml
    • 特异性抗原位于human物种的caspase-3 p17的内部区域内的氨基酸50-86之间
    • 抗-caspase-3 p17 抗体 (D-12) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 caspase-3 p17 and full length procaspase-3
    • 抗-caspase-3 p17 抗体 (D-12) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 关于如何获取caspase-3 p17 (D-12): sc-373730免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred secondary detection reagent for caspase-3 p17 Antibody (D-12) for WB and IHC(P) applications. This reagent is now offered in a bundle with caspase-3 p17 Antibody (D-12) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

What application is the blocking peptide sc-373730 P appropriate for?

Asked by: TinTin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Quite good antibody 1:200 diluted for wb of rat protein ,the band is at 15kd
Date published: 2019-06-17
Rated 3 out of 5 by from It was great It was great but doesn't work well after a new batch.
Date published: 2018-06-29
Rated 5 out of 5 by from caspase-3 p17 Western blot of mouse primary cerebellar neuron conjugated with caspase-3 p17 (1:1000). Protein concentration 30 µg per lane. Strong expression with low background.
Date published: 2018-03-09
Rated 5 out of 5 by from Good production we used TG mice Hip as sample,and the result as we expected,
Date published: 2018-03-01
Rated 4 out of 5 by from Weak signal at 17kDa on Western Blot. Detects doublet around 36kDa (uncleaved form) a weakly detects 17kDa band. Needs further optimizing.
Date published: 2018-01-31
Rated 4 out of 5 by from good antibody I have used the caspase-3 p17 antibody, the total band is so clear and the p17 band is less than the total band.
Date published: 2017-10-06
Rated 3 out of 5 by from weak bands can be seen you can see weak bands with the antibody. The Ph value of 1*TBS buffer is 7.45. I haven't try a buffer with lower Ph value.
Date published: 2017-09-30
Rated 5 out of 5 by from Great for early apoptosis detection Got this Ab as a sample, and tested on HEK cells, seems to work great.
Date published: 2017-09-25
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