caspase-3 p17抗体 (B-4) 推荐用于 human 来源的p17 subunit and full length precursor of caspase-3 WB, IP, IF, IHC(P) 和 ELISA检测

caspase-3 p17 抗体 (B-4): sc-271028

caspase-3 p17抗体 (B-4) is rated 4.9 out of 5 by 11.
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说明书

    • caspase-3 p17抗体(B-4)是小鼠单克隆IgG2a κ, 在53篇文献中引用,规格为200 µg/ml
    • 免疫原氨基酸序列56-104位于human物种的caspase-3 p17的N-terminus附近
    • caspase-3 p17 抗体 (B-4) 推荐用于WB, IP, IF, IHC(P) 和 ELISA,检测human 来源的 p17 subunit and full length precursor of caspase-3
    • 抗-caspase-3 p17 抗体 (B-4) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 关于如何获取caspase-3 p17 (B-4): sc-271028免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgG Fc BP-HRP (小鼠IgG Fc 结合蛋白偶联辣根过氧化物酶(HRP))m-IgGκ BP-HRP (小鼠IgGκ 结合蛋白偶联辣根过氧化物酶(HRP)) 是 caspase-3 p17 Antibody (B-4) for WB and IHC(P) 的首选应用二抗。这些试剂现在以套装形式提供caspase-3 p17 Antibody (B-4)。 (见下方订购信息).

Can this antibody recognize Caspase 3 after being cleaved? The size is about 17kDa.

Asked by: lidi
Thank you for your question. This monoclonal antibody is recommended for detection of the cleaved p17 subunit and full length precursor of caspase-3 of human origin. There is Western blot data on our website showing detection of the cleaved caspase-3 p17 in Staurosporine treated HeLa whole cell lysates.
Answered by: Technical Support
Date published: 2019-01-25

Is there a recommended positive control for this Ab?

Asked by: Rachie
Thank you for your question. We recommend using the following positive controls with this caspase-3 p17 Antibody (B-4): sc-271028: Untreated (sc-2200) and Staurosporine (sc-3510) treated HeLa (sc-24698) or Jurkat (sc-24719) whole cell lysates for detection of procaspase-3 and cleaved caspase-3 p17 expression in Western blot. Untreated (sc-2239) and Kendomycin (sc-202196) treated U-937 whole cell lysates for detection of procaspase-3 and cleaved caspase-3 p17 expression in Western blot. CCRF-CEM (sc-2225), Jurkat (sc-2204), K-562 (sc-2203), and Hep G2 (sc-2227) whole cell lysates for detection of procaspase-3 expression in Western blot.
Answered by: Technical Support
Date published: 2017-05-16

Can this antibody also recognize the p12 subunit of caspase 3?

Asked by: AbPolly
Thank you for your question. Our caspase-3 p17 sc-271028 is specific to the precursor form of caspase-3 and the p17 subunit, and will not detect the p12 subunit.
Answered by: Technical Support 2
Date published: 2017-01-24
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Rated 5 out of 5 by from Very good in Western blotting Pro and cleaved forms of caspase-3 were detectable in CCCP-treated K562 cells by Western blotting.
Date published: 2019-12-24
Rated 5 out of 5 by from Works Perfectly on WB This antibody works perfectly on WB analysis, incubation Overnight at 1/2000 in non dry fat milk
Date published: 2018-06-02
Rated 5 out of 5 by from Perfect clean detection 33 kDa (pro-caspase) band This Ab lit up in the correct Mw range (33 kDa) very quickly and cleanly. Unfortunately, we were unable to see the p17 fragment. We're currently optimising our cell culture incubations to detect this. Otherwise, very clean WB from this Ab. Would recommend. Conditions: 40 µg protein, 1º Ab 1/200, overnight at RT, 2º Ab, 1/10,000 m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) #sc-516102, 2 hrs RT. Imaging: ECL.
Date published: 2017-05-09
Rated 5 out of 5 by from works well Western blotting detection of human cancer cells, get the right band.
Date published: 2017-04-05
Rated 5 out of 5 by from Great for Immunoperoxidase This is antibody using Immunoperoxidase staining of human duodenum tissue, and it's very specific and clear.
Date published: 2017-03-28
Rated 5 out of 5 by from WB: clean Worked great on both our human samples for WB, 1:1000 dilution, very well established antibody.
Date published: 2017-02-12
Rated 5 out of 5 by from Works for detection of p17 subunit The (B-4) clone works for detection of the p17 subunit by WB in Staurosporine treated samples.
Date published: 2017-01-05
Rated 5 out of 5 by from Wonderful The memberane was blocked in 5% skim milk and probed with the caspase-3 antibody,the result is as:
Date published: 2016-11-24
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