caspase-3抗体 (E-8) 推荐用于 和 human 来源的caspase-3 and full length procaspase-3 WB, IP, IF, IHC(P), FCM 和 ELISA检测

caspase-3 抗体 (E-8): sc-7272

caspase-3抗体 (E-8) is rated 4.3 out of 5 by 48.
  • y_2021, m_5, d_12, h_17
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.15
  • cp_1, bvpage1
  • co_hasreviews, tv_1, tr_47
  • loc_zh_CN, sid_7272, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 115ms
  • REVIEWS, PRODUCT
5
31
4
10
3
3
2
0
1
4

说明书

    • 抗-caspase-3 抗体 E-8 是小鼠单克隆 IgG2a κ,caspase-3抗体, 在607篇文献中引用,规格为200 µg/ml
    • 免疫1-277氨基酸,对应于human来源的caspase-3的全长前体形式
    • caspase-3 抗体 (E-8) 推荐用于WB, IP, IF, IHC(P), FCM 和 ELISA,检测human 来源的 caspase-3 and full length procaspase-3
    • 抗-caspase-3 抗体 (E-8) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 还可偶联TRITC 以及 Alexa Fluor® 405 用于IF, IHC(P) 和 FCM
    • 关于如何获取caspase-3 (E-8): sc-7272免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgG Fc BP-HRP (小鼠IgG Fc 结合蛋白偶联辣根过氧化物酶(HRP)) 是 caspase-3 Antibody (E-8) for WB and IHC(P) 的首选应用二抗。这个产品以套装形式提供 caspase-3 Antibody (E-8) (参照下方订购信息).

Which species has this antibody been applied to? Which species does it work for, and which not?

Asked by: DABB
Thanks for your question. The caspase-3 (E-8) antibody is recommended for detection of caspase-3 and full length procaspase-3 of human origin by WB, IP, IF, IHC(P), FCM and ELISA. Please contact Technical service for further support.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

I used m-IgGκ BP-HRP: sc-516102 as the secondary antibody, but the bands didn`t existed. The reason is that m-IgGκ BP-HRP: sc-516102 didn`t binded to caspase-3  (E-8): sc-7272, isn`t it ?

Asked by: jmjflying918
Thank you for your question. There are several possibilities that could explain a negative result in Western blot. In this case, one possibility might be that this primary antibody, caspase-3 Antibody (E-8) is a mouse monoclonal with a lambda light chain. If that is the case, then m-IgGκ BP-HRP: sc-516102 would not work. Instead, we would recommend using m-IgGλ BP-HRP: sc-516132 which specifically binds to the lambda light chain of mouse immunoglobulins. We are testing sc-7272 now and will update our product information shortly to specify whether or not this antibody has a kappa or lambda light chain. In the meantime, please contact our Technical Support Department and we would be happy to continue troubleshooting the antibody. Please call our Asian Support office toll-free at 800-820-8626, email [email protected], or contact us by live chat.
Answered by: Technical Support
Date published: 2017-01-06

How should I go about double staining a tissue with this antibody if my other primary antibody is also anti-mouse?

Asked by: Trav11
In this case, we recommend using our directly conjugated mouse monoclonal primary antibodies. Currently, caspase-3 Antibody (E-8): sc-7272 is available directly conjugated to HRP (sc-7272 HRP), phycoerythrin (sc-7272 PE), fluorescein (sc-7272 FITC), Alexa Fluor® 488 (sc-7272 AF488), Alexa Fluor® 647 (sc-7272 AF647), TRITC (sc-7272 TRITC, 200 µg/ml) or Alexa Fluor® 405 (sc-7272 AF405, 100 µg/2 ml).
Answered by: Tech Service 8
Date published: 2016-12-28
  • y_2021, m_5, d_12, h_17CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.15
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_3
  • loc_zh_CN, sid_7272, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 129ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Ótimo produto Pedi uma amostra de casp-3 e funcionou perfeitamente.
Date published: 2021-01-08
Rated 4 out of 5 by from buon anticorpo Questo anticorpo dà un bel segnale, netto e specifico.
Date published: 2020-11-04
Rated 1 out of 5 by from No detectable in adipose tissue of mice Caspase3 protein was no detectable in subcutaneous white adipose tissue at the position of proper size.
Date published: 2020-01-27
Rated 4 out of 5 by from Excellent. This antibody works great for western blotting.Excellent.
Date published: 2019-07-30
Rated 1 out of 5 by from Unspecific bands In WB, we got multiple bands, non of which was at the expected size.
Date published: 2018-03-15
Rated 3 out of 5 by from Band intensity in week Antibody worked but the band intensity is week. maybe I should loaded more protein (usually I load 40-50uG).
Date published: 2018-02-01
Rated 5 out of 5 by from Works great for FFPE IHC We got this as a sample. This is an easy, nice working antibody to use. HIER sodium citrate pH 6.0, 1:100 on human heart, and appendix. Looks great!
Date published: 2018-01-04
Rated 5 out of 5 by from This antibody is very good for Western Blot This antibody is very good for Western Blot,and I am so glad to use it!
Date published: 2017-12-06
  • y_2021, m_5, d_12, h_17
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.15
  • cp_1, bvpage1
  • co_hasreviews, tv_1, tr_47
  • loc_zh_CN, sid_7272, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 7ms
  • REVIEWS, PRODUCT

圣克鲁斯生物技术在生物医药研究市场的产品开发方面处于世界领先地位。欢迎 致电我们的免费电话(021) 800-820-8626。 版权 ©, 2007-2021圣克鲁斯生物技术。版权所有。“Santa Cruz Biotechnology”,圣克鲁斯生物技 术的 商标,“Santa Cruz Animal Health”,“San Juan Ranch”,“Supplement of Champions”,San Juan Ranch的商标,“Ultracruz”,“Chemcruz”,“Immunocruz”,“Exactacruz”和“EZ Touch”都是圣克鲁斯生物技术的注册商标。所有的商标都归其各自拥有者所有。