caspase-3抗体 (7.1.44) 推荐用于 mouse, rat 和 human 来源的caspase-3 WB, IP, IF, IHC(P) 和 ELISA检测

caspase-3 抗体 (7.1.44): sc-56051

caspase-3抗体 (7.1.44) is rated 4.0 out of 5 by 1.
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说明书

    • caspase-3抗体(7.1.44)是小鼠单克隆IgG2a κ, 在2篇文献中引用,规格为200 µg/ml
    • 免疫human来源的caspase-3全长
    • 推荐用于 mouse, rat 和 human 来源的caspase-3 WB, IP, IF, IHC(P) 和 ELISA检测
    • 看 caspase-3 p11 (C-6): sc-271759,可查看有标记的 caspase-3 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred secondary detection reagent for caspase-3 Antibody (7.1.44) for WB and IHC(P) applications. This reagent is now offered in a bundle with caspase-3 Antibody (7.1.44) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

How much sample do you recommend loading on the gel for Western blot detection using caspase-3 (7.1.44): sc-56051?

Asked by: cjMara
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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Rated 4 out of 5 by from Good Western results with HT Good Western results with HT-29 and HCT-116 cells. -SCBT Publication Review
Date published: 2011-12-15
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