caspase-3抗体 (4.1.18) 推荐用于 mouse, rat 和 human 来源的caspase-3 WB, IP, IF, IHC(P) 和 ELISA检测

caspase-3 抗体 (4.1.18): sc-65497

caspase-3抗体 (4.1.18) is rated 5.0 out of 5 by 3.
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说明书

    • caspase-3抗体(4.1.18)是小鼠单克隆IgG2a κ, 在32篇文献中引用,规格为200 µg/ml
    • 免疫human来源的caspase-3全长
    • 推荐用于 mouse, rat 和 human 来源的caspase-3 WB, IP, IF, IHC(P) 和 ELISA检测
    • 看 caspase-3 p11 (C-6): sc-271759,可查看有标记的 caspase-3 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
    • m-IgG Fc BP-HRP (小鼠IgG Fc 结合蛋白偶联辣根过氧化物酶(HRP)) 是 caspase-3 Antibody (4.1.18) for WB and IHC(P) 的首选应用二抗。这个产品以套装形式提供 caspase-3 Antibody (4.1.18) (参照下方订购信息).

Does it function to detect cleaved caspase 3?

Asked by: Dr Inês
Thank you for your question. We have used this mouse monoclonal antibody, caspase-3 (4.1.18): sc-65497, to successfully detect procaspase-3. We do not yet have data with this clone detecting cleaved caspase-3, but the optimal conditions for detection of cleaved caspase-3 may not yet be determined. This antibody has been cited in the literature at least 14 times. We would recommend consulting some of the product citations to see if anyone has used this clone for detection of the cleaved caspase-3.
Answered by: Technical Support
Date published: 2017-06-01

I am using caspase-3 (4.1.18): sc-65497 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Dr Ninau Qelp
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from Perfect I used this AB in WB and work perfectly, clear and nice bands.
Date published: 2019-05-13
Rated 5 out of 5 by from Well-rounded antibody for caspase-3 Works great for both WB and IHC on paraffin embedded tissue sections.
Date published: 2017-01-18
Rated 5 out of 5 by from Good for Western with human cell lysates Good for Western with human cell lysates -SCBT QC
Date published: 2014-12-04
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