Calponin 1/2/3抗体 (G-10) 推荐用于 Calponin 1 of mouse, rat and human origin, and Calponin 2 and Calponin 3 of human origin WB, IP, IF, IHC(P) 和 ELISA检测

Calponin 1/2/3 抗体 (G-10): sc-136987

Calponin 1/2/3抗体 (G-10) is rated 4.9 out of 5 by 8.
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说明书

    • 抗-Calponin 1/2/3 抗体 G-10 是小鼠单克隆 IgG1 κ,Calponin 1/2/3抗体, 在23篇文献中引用,规格为200 µg/ml
    • raised against amino acids 1-297 representing full length Calponin 1 of human origin
    • Calponin 1/2/3 抗体 (G-10) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测 Calponin 1 of mouse, rat and human origin, and Calponin 2 and Calponin 3 of human origin
    • 抗-Calponin 1/2/3 抗体 (G-10) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 关于如何获取Calponin 1/2/3 (G-10): sc-136987免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • 目前,我们还没有完成Calponin 1/2/3 抗体 (G-10)的首选二抗检测试剂的鉴定。这项工作正在进行中。
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Can Calponin 1/2/3 (G-10): sc-136987 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: DefinitelyNotMatt
Thank you for your question. Yes, Calponin 1/2/3 (G-10): sc-136987 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 5 out of 5 by from Good results on mouse tissue IF(P) We tried this antibody on paraffin sections of paraformaldehyde fixed mouse embryonic gonads and had good results (pH6 antigen retrieval).
Date published: 2018-03-20
Rated 5 out of 5 by from Pretty good for CNN1 in IF staining I used this as 1:100 in cardiomyocytes for IF staining, shows very clear structure of the myofilament, thanks for Santa.
Date published: 2017-10-24
Rated 4 out of 5 by from just ok thanks FOR the sample , BUT the bind is not clear enough
Date published: 2017-07-22
Rated 5 out of 5 by from Strong and specific antibody by WB This wonderful antibody is sure to produce the desired results in all standard applications. Reactivity with mouse, rat, and human gives it great versatility.
Date published: 2017-04-06
Rated 5 out of 5 by from very well! I applied this small sample half a month ago, the signal is clear and strong, good!
Date published: 2016-11-08
Rated 5 out of 5 by from Western blot Single clear, distinct band (used at 1:500 dilution) at ~34kDa in myofibroblasts.
Date published: 2016-10-28
Rated 5 out of 5 by from Produced great immunofluorescence cytoskeletal Produced great immunofluorescence cytoskeletal staining in formalin-fixed HepG2 cells. -SCBT QC
Date published: 2015-05-08
Rated 5 out of 5 by from Clean bands in western blot analysis of Calponin Clean bands in western blot analysis of Calponin 1/2/3 expression in 293T and JEG-3 whole cell lysates. -SCBT QC
Date published: 2015-03-26
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