BRCA1 CRISPR 质粒 (h) human BRCA1特异的CRISPR/Cas9 KO质粒,HDR质粒,双切口酶质粒,CRISPR激活质粒和慢病毒激活颗粒

BRCA1 CRISPR 质粒 (h)

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说明书

    • 目标种属:human; 用于相应的mouse产品,查看BRCA1 CRISPR Plasmids (m)
    • CRISPR/Cas9 KO质粒包含源自GeCKO (v2)库的BRCA1特异的20nt向导RNA序列
    • 对于CRISPR基因敲除,gRNA 序列指导Cas9蛋白诱导基因组DNA中位点特异的双链断裂(DSB)
    • 可提供目标特异的基因敲除和激活的CRISPR质粒。请参考下面标签中详细的产品信息
    • 基因的敲除或者激活可以使用 BRCA1 Antibody (D-9): sc-6954分析
    • 除慢病毒激活颗粒外,其他产品均为转染即用型纯化DNA质粒。慢病毒激活颗粒预包被成慢病毒颗粒的形式,适用于难被转染的细胞

基因信息

种属基因名称基因ID染色质位置亚型(mRNA)即位号码蛋白质即位号码OMIM™ 号码
HumanBRCA167217q21.31NM_007294, NM_007297, NM_007298, NM_007299, NM_007300, NM_007295, NM_007296, NM_007301, NM_007302, NM_007303, NM_007305, NM_007306P38398113705

We just bought the BRCA1 CRISPR/Cas9 KO (h) plasmid. However, the transfection efficiency is only ~1%. We need do some trouble shoots. Do you have the plasmid sequence available? What is the antibiotic selection? Thanks advance for your answer.

Asked by: yangcs01
Thank you for your question. Please contact our technical support group by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com, and we would be happy to provide you with this information and help you troubleshoot the CRISPR.
Answered by: Tech Service
Date published: 2017-08-14
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