Bicine (cell culture suitable) CAS: 150-25-4
MF: C6H13NO4
MW: 163.17
A zwitterionic amino acid buffer agent used for low temperature work.

Bicine (cell culture suitable) (CAS 150-25-4)

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备用名: N,N-Bis-(2-hydroxyethyl)-glycine
应用; Bicine (cell culture suitable) is a zwitterionic amino acid buffer agent used for low temperature work
CAS号码: 150-25-4
分子量: 163.17
分子式: C6H13NO4
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).
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Bicine is a zwitterionic amino acid buffer, active in the pH 7.6-9.0 range (pKa of 8.26 at 25°C). Recommended buffer for low temperature biochemical work. Bicine is used for the preparation of stable substrate solution for serum guanase determination. The use of bicine in a thin layer ion exchange chromatography method for protein resolution has been published. Bicine has been utilized in peptide and protein crystallization. A kinetic study of a quaternary transition-state analogue complex of creatine kinase used bicine in the reaction buffer. A multiphasic buffer system for SDS-PAGE of proteins and peptides that incorporates bicine has been described.

外观 :
Powder
物理状态 :
Solid
溶解度 :
Soluble in water (163.17 g/l) at 20 °C, ethanol (slightly soluble), ether (slightly soluble), and chloroform (slightly soluble).
保存 :
Store at room temperature
熔点 :
190° C (dec.)
沸点 :
361.50-415.50° C at 760 mmHg (Predicted)
密度 :
1.31 g/cm3 (Predicted)
折射率 :
n20D 1.53 (Predicted)
pK值 :
pKa: 8.3 (0.1 M, 25° C)
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
德国水公害等级 :
3
RTECS :
MB9700000
PubChem CID :
默克索引 :
14: 1201
MDL 号码 :
MFCD00004295
EC号码 :
205-755-1
Beilstein 注册 :
1769362
SMILES :
C(CO)N(CCO)CC(=O)O

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