Benzonase® Nuclease 生物活性蛋白:适用于需要完整消化核酸的核酸内切酶

Benzonase® Nuclease (CAS 9025-65-4)

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同义词: BenzNuclease
应用; Benzonase® Nuclease is an endonuclease ideal for applications where complete digestion of nucleic acids is desirable
CAS号码: 9025-65-4
纯度: ≥90%
* 参考分析证明 大量特定数据 (包括水 含量).
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Benzonase® Nuclease is a solubilized version of Benzonase® Nuclease Lyophilized Powder (sc-391121). The Benzonase® enzyme is a genetically engineered endonuclease from Serratia marcescens. Benzonase® Nuclease, also called Golden nuclease, is a highly useful endonuclease for removal of nucleic acids from recombinant proteins and protein fragments, and is also used for the reduction of viscosity in protein extracts.

Benzonase® Nuclease is a genetically engineered endonuclease from Serratia marcescens. Benzonase® Nuclease, also called Golden nuclease, is a highly useful endonuclease for removal of nucleic acids from recombinant proteins and protein fragments, and is also used for the reduction of viscosity in protein extracts.

The enzyme completely digests nucleic acids to 5′-monophosphate terminated oligonucleotides 2–5 bases in length. Although the nuclease is capable of cleavage at nearly all positions along a nucleic acid chain, sequence-dependent preferences have been demonstrated. The enzyme prefers GC-rich regions in dsDNA while avoiding d(A)/d(T)-tracts. Benzonase is ideal for a wide variety of applications where complete digestion of nucleic acids is desirable. Digests native or heat-denatured DNA and RNA. This can also be used for the removal of nucleic acid from protein samples.

Unit definition:
One unit of Benzonase Nuclease is defined as the amount of enzyme that causes a ΔA260 of 1.0 in 30 min, which corresponds to complete digestion of 37 μg of DNA. Note that 1 KU = 1000 units.

Benzonase is a registered trademark of Merck KGaA.


参考文献

1. Miller, M.D., et al. 1994. Nat. Struct. Biol. 1: 461-468. PMID: 7664065

2. Friedhoff, P., et al. 1994. Nucleic Acids Res. 22: 3280-3287. PMID: 8078761

3. Ball, T.K., et al. 1992. Nucleic Acids Res. 20: 4971-4974. PMID: 1329033

4. Ball, T.K., et al. 1987. Gene. 57: 183-192. PMID: 3319779

5. Janning, P., et al. 1994. Rapid Commun. Mass Spectrom. 8: 1035-1040. PMID: 7696699

6. Molloy, M.P., et al. 1998. Electrophoresis. 19: 837-844. PMID: 9629924

7. Herbert, B.R., et al. 1998. Electrophoresis. 19: 845-851. PMID: 9629925

用法 :
The activity of any unknown nuclease can be determined from a single measurement by means of the standard curve. The specific activity of Benzonase® Nuclease is 1.3×106 ~ 1.5×106 unit/mg protein.
规划 :
≥250 units/µL supplied in solution containing 20 mM Tris HCl (pH 8.0), 2 mM MgCl2, 20 mM NaCl and 50% glycerol.
物理状态 :
Liquid
来源于 :
Recombinant protein expressed in Escherichia coli
保存 :
Store at -20° C
仅供科研使用。不可用于诊断或治疗。
德国水公害等级 :
1
MDL 号码 :
MFCD00131010
EC号码 :
231-791-2

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PMID: # 31263572  Keskitalo, S.|Haapaniemi, EM.|Glumoff, V.|Liu, X.|Lehtinen, V.|Fogarty, C.|Rajala, H.|Chiang, SC.|Mustjoki, S.|Kovanen, P.|Lohi, J.|Bryceson, YT.|Seppänen, M.|Kere, J.|Heiskanen, K.|Varjosalo, M.| et al. 2019. NPJ Genom Med. 4: 14.

PMID: # 30692695  Charlesworth, CT.|Deshpande, PS.|Dever, DP.|Camarena, J.|Lemgart, VT.|Cromer, MK.|Vakulskas, CA.|Collingwood, MA.|Zhang, L.|Bode, NM.|Behlke, MA.|Dejene, B.|Cieniewicz, B.|Romano, R.|Lesch, BJ.|Gomez-Ospina, N.|Mantri, S.|Pavel-Dinu, M.|Weinberg, KI.|Porteus, MH.| et al. 2019. Nat. Med. 25: 249-254.

PMID: # 31135337  Bigot, N.|Day, M.|Baldock, RA.|Watts, FZ.|Oliver, AW.|Pearl, LH.| et al. 2019. Elife. 8:

PMID: # 31163614  Villafañez, F.|Gottifredi, V.|Soria, G.| et al. 2019. High Throughput. 8:

PMID: # 29565900  van Rooden, EJ.|Florea, BI.|Deng, H.|Baggelaar, MP.|van Esbroeck, ACM.|Zhou, J.|Overkleeft, HS.|van der Stelt, M.| et al. 2018. Nat Protoc. 13: 752-767.

PMID: # 30205044  Archer, TC.|Ehrenberger, T.|Mundt, F.|Gold, MP.|Krug, K.|Mah, CK.|Mahoney, EL.|Daniel, CJ.|LeNail, A.|Ramamoorthy, D.|Mertins, P.|Mani, DR.|Zhang, H.|Gillette, MA.|Clauser, K.|Noble, M.|Tang, LC.|Pierre-François, J.|Silterra, J.|Jensen, J.|Tamayo, P.|Korshunov, A.|Pfister, SM.|Kool, M.|Northcott, PA.|Sears, RC.|Lipton, JO.|Carr, SA.|Mesirov, JP.|Pomeroy, SL.|Fraenkel, E.| et al. 2018. Cancer Cell. 34: 396-410.e8.

PMID: # 29303417  Farrokhi, A.|Pakyari, M.|Nabai, L.|Pourghadiri, A.|Hartwell, R.|Jalili, R.|Ghahary, A.| et al. 2018. Tissue Eng Part A. 24: 955-967.

PMID: # 30154153  Högström, J.|Heino, S.|Kallio, P.|Lähde, M.|Leppänen, VM.|Balboa, D.|Wiener, Z.|Alitalo, K.| et al. 2018. Cancer Res. 78: 5820-5832.

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