ATP5B抗体 (10) 推荐用于 mouse, rat, human 和 canine 来源的ATP5B WB, IP 和 IF检测

ATP5B 抗体 (10): sc-135903

ATP5B抗体 (10) is rated 4.0 out of 5 by 4.
  • y_2020, m_10, d_29, h_16
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.12
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_4
  • loc_zh_CN, sid_135903, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 124ms
  • REVIEWS, PRODUCT
5
0
4
4
3
0
2
0
1
0

说明书

    • ATP5B 抗体 10 是小鼠单克隆 IgG1,提供 50 µg/0.5 ml
    • 免疫human物种的F1-ATPase的氨基酸428-539
    • 推荐用于 mouse, rat, human 和 canine 来源的ATP5B WB, IP 和 IF检测

I am using ATP5B (10): sc-135903 for immunofluorescence, what dilution of this antibody should I use?

Asked by: Professor Griffin
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
  • y_2020, m_10, d_29, h_16CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.12
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_zh_CN, sid_135903, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 166ms
  • QUESTIONS, PRODUCT
Rated 4 out of 5 by from works well 1:500 dilution works well in HEK293 cells, the blot shows enrichment of it in mito fraction in last three wells
Date published: 2018-09-29
Rated 4 out of 5 by from Worked well It worked well in the mitochondria fraction of Hs578t and MDA-MB-157 cell lines ( 2% milk-1:1000).
Date published: 2017-10-31
Rated 4 out of 5 by from Strong band seen in WB using Jurkat whole Strong band seen in WB using Jurkat whole cell lysate. -SCBT QC
Date published: 2015-03-13
Rated 4 out of 5 by from Produced good immunofluorescence cytoplasmic Produced good immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-01-21
  • y_2020, m_10, d_29, h_16
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.12
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_4
  • loc_zh_CN, sid_135903, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 11ms
  • REVIEWS, PRODUCT

圣克鲁斯生物技术在生物医药研究市场的产品开发方面处于世界领先地位。欢迎 致电我们的免费电话1-800-457-3801。 版权 ©, 2007-2020圣克鲁斯生物技术。版权所有。“Santa Cruz Biotechnology”,圣克鲁斯生物技 术的 商标,“Santa Cruz Animal Health”,“San Juan Ranch”,“Supplement of Champions”,San Juan Ranch的商标,“Ultracruz”,“Chemcruz”,“Immunocruz”,“Exactacruz”和“EZ Touch”都是圣克鲁斯生物技术的注册商标。所有的商标都归其各自拥有者所有。