Ameloblastin抗体 (H-2) 推荐用于 human 来源的Ameloblastin WB, IP, IF, IHC(P) 和 ELISA检测

Ameloblastin 抗体 (H-2): sc-271012

Ameloblastin抗体 (H-2) is rated 3.5 out of 5 by 4.
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说明书

    • 抗-Ameloblastin 抗体 H-2 是小鼠单克隆 IgG1 κ,Ameloblastin抗体, 在2篇文献中引用,规格为200 µg/ml
    • raised against amino acids 148-447 mapping at the C-terminus of Ameloblastin of human origin
    • Ameloblastin 抗体 (H-2) 推荐用于WB, IP, IF, IHC(P) 和 ELISA,检测human 来源的 Ameloblastin
    • 抗-Ameloblastin 抗体 (H-2) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 关于如何获取Ameloblastin (H-2): sc-271012免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP 是 Ameloblastin Antibody (H-2) for WB and IHC(P)首选应用二抗。这些试剂现在以套装形式提供Ameloblastin Antibody (H-2) (见下方订购信息).
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Can Ameloblastin (H-2): sc-271012 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Randy McDonald
Thank you for your question. Yes, Ameloblastin (H-2): sc-271012 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 3 out of 5 by from Works for Westerns not for IHC in mouse tissue This antibody worked for recombinant murine and native porcine Ambn Western blots but did not work in IHC staining of mouse sections. Previous M300 ameloblastin antibody (rabbit polyclonal) used to work very well (1:500 dilution for IHC) and N18 ameloblastin ab (goat polyclonal) worked at higher concentrations (1:100). I have tried both concentrations for H2 and it did not work.
Date published: 2018-11-12
Rated 1 out of 5 by from Doesn't work in rat and mouse ameloblast SC's previous polyclonal AMBN antibody worked great for me, however this new mouse mono Ab doesn't seem to be equivalent. It doesn't work in my immunofluorescent staining of rat and mouse ameloblasts (paraffin embeding and enzyme/citrate heat retrieval).
Date published: 2017-07-05
Rated 5 out of 5 by from Produced beautiful immunoperoxidase cytoplasmic Produced beautiful immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human kidney tissue. -SCBT QC
Date published: 2015-02-07
Rated 5 out of 5 by from Nice Western Blot data of human recombinant Nice Western Blot data of human recombinant Ameloblastin fusion protein. -SCBT QC
Date published: 2014-08-04
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