5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside CAS: 7240-90-6
MF: C14H15BrClNO6
MW: 408.63

5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside (CAS 7240-90-6)

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备用名: X-Gal; BCIG; 5-Bromo-4-chloro-3-indolyl-β-D-galactoside; X Gal
应用; 5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside is a chromogenic histochemical substrate for β-galactosidase
CAS号码: 7240-90-6
纯度: ≥99%
分子量: 408.63
分子式: C14H15BrClNO6
仅供科研使用。不可用于诊断或治疗。
* 参考分析证明 大量特定数据 (包括水 含量).
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5-Bromo-4-chloro-3-indolyl b-D-galactopyranoside (X-Gal) is a histochemical substrate for β-galactosidase. X-Gal is cleaved by β-galactosidase to yield an insoluble blue preceipitate. X-Gal is used to detect DNA insertions into the lacZ region of plasmid DNA, which causes the loss of β-galactosidase activity. Also used in conjunction with IPTG for detection of b-Galactosidase activity in bacterial colonies in a colorimetric assay.

外观 :
Crystalline powder
物理状态 :
Solid
溶解度 :
Soluble in DMF (5% w/v), DMSO (20 mg/ml), and water (partly miscible).
保存 :
Store at -20° C
熔点 :
236-238° C (dec.)
沸点 :
698.00° C at 760 mmHg (Predicted)
光学活性 :
α20/D -60°, c = 1 in 50% DMF; α20/D -66°°, c = 1 in DMF
仅供科研使用。不可用于诊断或治疗。
德国水公害等级 :
3
PubChem CID :
默克索引 :
14: 10074
MDL 号码 :
MFCD00005666
EC号码 :
230-640-8
Beilstein 注册 :
1402009
SMILES :

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PMID: # 29533388  Maiarù, M.|Morgan, OB.|Mao, T.|Breitsamer, M.|Bamber, H.|Pöhlmann, M.|Schmidt, MV.|Winter, G.|Hausch, F.|Géranton, SM.| et al. 2018. Pain. 159: 1224-1234.

PMID: # 28553941  Riglar, DT. et al. 2017. Nat. Biotechnol. 35: 653-658.

PMID: # 26133793  Deng, L. et al. 2015. Brain Res. 1622: 186-95.

PMID: # 32783031  STAR Protoc.

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