TASK-2 siRNA and shRNA Plasmids (h)

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Species	Gene Name	Gene ID	Chromosome Location	Isoform (mRNA) Accession #	Protein Accession #	OMIM™ Number
Human	KCNK5	8645	6p21.2	XM_005249456, XM_006715235, NM_003740	O95279	603493

Beskrivning:

See Protocol Online.

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20 µl of each primer set is provided at a concentration of 10 µM
TASK-2 Primer (h) includes A set (forward & reverse) and B set (forward & reverse) primers
Primer sets are recommended for semi-quantitative nested reverse transcriptase PCR

See Protocol Online.

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Beskrivning:

20 µg, up to 20 transfections
shRNA Plasmids are provided as a pool of three to five lentiviral vector plasmids which each encode a target specific 19-25 nt shRNA with a 6 bp loop
provided as transfection-ready purified plasmid DNA
After transfection, cells stably expressing shRNA can be selected by puromycin treatment
Tillgängliga antikroppar: TASK-2: sc-28632

See Protocol Online.

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Beskrivning:

200 µl viral stock containing 10⁶ infectious lentiviral transducing particles per ml, sufficient for 10-20 transductions
Lentiviral Particles are provided as transduction-ready viruses for targeted gene silencing in mammalian cells (human or mouse)
The Lentiviral Particles generally contain three to five expression constructs, each construct encoding a target specific 19-25 nt shRNA with a 6 bp loop
After transduction, cells stably expressing shRNA can be selected by puromycin treatment
Lentiviral particles can be employed in standard Biosafety Level 2 tissue culture facilities (and should be treated with the same level of caution as with any other potentially infectious reagent). Lentiviral particles are replication-incompetent and are designed to self-inactivate after transduction and integration of shRNA constructs into genomic DNA of target cells.

See Protocol Online.

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