Protein L-Agarose: sc-2336

Molecular purification methods such as immunoprecipitation (IP), co-IP and chromatin IP are important tools in studying lowly expressed proteins, protein-protein interactions or protein-nucleic acid interactions. Santa Cruz Biotechnology offers a comprehensive array of research support products for purification techniques. Protein L Agarose is a recommended specialty reagent used for isolating immunoglobulins from mammalian species. Protein L is an immunoglobulin binding protein that was originally derived from Peptostreptococcus magnus. Unlike Protein A and Protein G, which bind primarily through Fc regions (i.e., heavy chain) of immunoglobilins, Protein L binds Igs through interactions with the light chains. Protein L Agarose is a recombinant Protein L that is linked to a high-quality Agarose resin, 4% B, and makes a premium product which provides specific binding to kappa light chains of mouse, rat and human IgG, mouse and human IgM, IgE and IgA proteins. Protein L is also recommended for binding single chain variable fragments (scFv) and Fab fragments containing kappa light chains which makes it suitable for purifying monoclonal antibodies from ascites or culture supernatant.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.