Phosphatase Inhibitor Cocktail A An inhibitor of L-isozymes of alkaline phosphatase and ser/thr protein phosphatases.

Phosphatase Inhibitor Cocktail A

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Alternativa namn: Phosphatase Inhibitor Cocktail I
Ansökan Phosphatase Inhibitor Cocktail A is an inhibitor of L-isozymes of alkaline phosphatase and ser/thr protein phosphatases
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).
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Phosphatase Inhibitor Cocktail A effectively inhibits L-isozymes of alkaline phosphatase and serine/threonine protein phosphatases, such as the PP1 (protein phosphatase 1) and PP2A) protein phosphatase 2a) proteins. Crude cell extracts contain a number of endogenous enzymes, such as proteases and phosphatases, which are capable of modifying the proteins present in the extract. The best way to improve the yield of intact proteins is to add inhibitors of these enzymes known to be present in the source material. Recommended for use with tissue and cell extracts, including extracts containing detergents.
Each vial contains 2.5 mM (-)-p-Bromotetramisole oxalate, 500 µM Cantharidin and 500 nM Microcystin-LR in 1 ml DMSO. Because this product is not available for sale outside of the United States, please see Phosphatase Inhibitor Cocktail C (sc-45065) as an alternate product.


Konferenser

1. Nishiwaki, S., et al. 1991. FEBS Lett. 279: 115-118. PMID: 1847341
2. Ilkovitch, D., et al. 2008. Cancer Res. 68: 2427-2435. PMID: 18381451
3. Wagner, M.W., et al. 2008. J. Biol. Chem. 283: 21382-21393. PMID: 18480061
4. Jain, M.K. 1982. Handbook of Enzyme Inhibitors. New York: John Wiley and Sons, Pg. 222, 334.

Usage :
• Recommended usage is 100-fold dilution.

• One ml of Phosphatase Inhibitor Cocktail A will inhibit phosphatase activities found in the 100,000 x g supernatant from human placenta, cow liver, rabbit muscle, A431 or Jurkat cell extracts at a protein concentration of approximately 5 mg/ml.

• One ml of Phosphatase Inhibitor Cocktail A solution is used to prepare 100 ml of supernatant that contains a maximum of 500 mg of protein. Therefore, 1 ml of cocktail should be added per 500 mg of protein extracted from the tissue used, or 1 ml of cocktail solution per 100 ml of extraction buffer.
Physical State :
Liquid
LAGRING :
Store at -20° C
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

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Certificate of Analysis

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Phosphatase Inhibitor Cocktail A  Produkt Citat

See how others have used Phosphatase Inhibitor Cocktail A. Click on the entry to view the PubMed entry .

Citations 1 to 10 of 56 total

PMID: # 33624556  Taylor, CM.|Kasztan, M.|Sedaka, R.|Molina, PA.|Dunaway, LS.|Pollock, JS.|Pollock, DM.| et al. 2021. Am J Physiol Regul Integr Comp Physiol. 320: R630-R640.

PMID: # 33500244  Duy, C.|Li, M.|Teater, M.|Meydan, C.|Garrett-Bakelman, FE.|Lee, TC.|Chin, CR.|Durmaz, C.|Kawabata, KC.|Dhimolea, E.|Mitsiades, CS.|Doehner, H.|D'Andrea, RJ.|Becker, MW.|Paietta, EM.|Mason, CE.|Carroll, M.|Melnick, AM.| et al. 2021. Cancer Discov.

PMID: # 34526526  Li, Y.|Mao, P.|Basenko, EY.|Lewis, Z.|Smerdon, MJ.|Czaja, W.| et al. 2021. Sci Rep. 11: 18393.

PMID: # 33441552  Wu, CL.|Dicks, A.|Steward, N.|Tang, R.|Katz, DB.|Choi, YR.|Guilak, F.| et al. 2021. Nat Commun. 12: 362.

PMID: # 31606082  Tiambeng, TN.|Tucholski, T.|Wu, Z.|Zhu, Y.|Mitchell, SD.|Roberts, DS.|Jin, Y.|Ge, Y.| et al. 2019. Methods Enzymol. 626: 347-374.

PMID: # 30942458  Ibrahim, SM.|Bakhashab, S.|Ilyas, AM.|Pushparaj, PN.|Karim, S.|Khan, JA.|Abuzenadah, AM.|Chaudhary, AG.|Al-Qahtani, MH.|Ahmed, F.| et al. 2019. Oncol. Rep. 41: 3179-3188.

PMID: # 30817799  Grant, MKO.|Handoko, M.|Rozga, M.|Brinkmalm, G.|Portelius, E.|Blennow, K.|Ashe, KH.|Zahs, KR.|Liu, P.| et al. 2019. PLoS ONE. 14: e0212815.

PMID: # 30979869  Yang, Y.|van der Klaauw, AA.|Zhu, L.|Cacciottolo, TM.|He, Y.|Stadler, LKJ.|Wang, C.|Xu, P.|Saito, K.|Hinton, A.|Yan, X.|Keogh, JM.|Henning, E.|Banton, MC.|Hendricks, AE.|Bochukova, EG.|Mistry, V.|Lawler, KL.|Liao, L.|Xu, J.|O'Rahilly, S.|Tong, Q.|, .|Inês Barroso, .|O'Malley, BW.|Farooqi, IS.|Xu, Y.| et al. 2019. Nat Commun. 10: 1718.

PMID: # 29491408  Shi, Q.|Viswanadhapalli, S.|Friedrichs, WE.|Velagapudi, C.|Szyndralewiez, C.|Bansal, S.|Bhat, MA.|Choudhury, GG.|Abboud, HE.| et al. 2018. Sci Rep. 8: 3781.

PMID: # 30050412  Shi, Q.|Saifetiarova, J.|Taylor, AM.|Bhat, MA.| et al. 2018. Front Cell Neurosci. 12: 201.

Citations 1 to 10 of 56 total
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