Anti-Hic-5 Antibody (C-6) is a mouse monoclonal IgG2b (kappa light chain) Hic-5 antibody provided at 200 µg/ml
specific for an epitope mapping between amino acids 409-434 near the C-terminus of Hic-5 of human origin
Anti-Hic-5 Antibody (C-6) is recommended for detection of Hic-5 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine and bovine
TransCruz reagent for ChIP application (sc-271353 X, 200 µg/0.1 ml)
Anti-Hic-5 Antibody (C-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Hic-5 (C-6): sc-271353.
m-IgG2b BP-HRP is the preferred secondary detection reagent for Hic-5 Antibody (C-6) for WB and IHC(P) applications. This reagent is now offered in a bundle with Hic-5 Antibody (C-6) (see ordering information below).
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
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Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
Hic-5 Antibody (C-6) is a high quality monoclonal Hic-5 antibody (also designated Hic-5 antibody) suitable for the detection of the Hic-5 protein of mouse, rat and human origin. Hic-5 Antibody (C-6) is available as both the non-conjugated anti-Hic-5 antibody form, as well as multiple conjugated forms of anti-Hic-5 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. In addition to paxillin, zysin, LPP, Ajuba and TRIP6, hydrogen-peroxide inducible clone 5 (Hic-5) is a member of the LIM family. Hic-5 contains four LIM motifs and seven zinc finger domains. In the cell, Hic-5 localizes to the nuclear matrix and focal adhesion complexes where the LIM domains mediate the interactions of Hic-5 with focal adhesions. Known also as transforming factor beta 1 induced transcript 1, Hic-5 shares extensive homology with the structural protein paxillin, which is involved in the regulation of focal adhesion dynamics. Hic-5 inhibits integrin-mediated cell spreading on Fibronectin by out competing paxillin for focal adhesion kinase and thereby preventing downstream signal transduction. Increased expression of Hic-5 leads to cellular senescence in developing fibroblasts. During myogenesis, expression of Hic-5 blocks differentiation and induces apoptosis of developing myoblasts. The gene encoding human Hic-5 maps to chromosome 16.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
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