FAT1 siRNA and shRNA Plasmids (m)

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- Target species: mouse; for corresponding human product, see FAT1 siRNA and shRNA Plasmids (h)
- Gene Silencers generally consist of pools of three to five target-specific 19-25 nucleotide sequences in length
- For independent verification of FAT1 gene silencing results, individual duplex components or plasmids are also available upon request
- Get details about si/shRNA Gene Silencers
- Gene silencers are intended for noncommercial and research use only. Click here for details.

Species	Gene Name	Gene ID	Chromosome Location	Isoform (mRNA) Accession #	Protein Accession #	OMIM™ Number
Mouse	Fat1	14107	8 B1.1	XM_006509267, XM_006509268, XM_006509270, XM_006509271, XM_006509272, XM_006509273, XM_006509274, XM_006509276, XM_006509277, XM_011242173, XM_017312564, NM_001081286	XP_006509330	N/A

Beskrivning:

10 µM, 50-100 transfections
siRNA products generally consist of pools of three to five target-specific 19-25 nt siRNAs designed to knockdown gene expression
for independent verification of FAT1 gene silencing results, individual siRNA duplex components are also available upon request
contact Technical Service for availability of control antibody
sqRT-PCR Primer also available: FAT1 (m)-PR: sc-145079-PR, 20 µl
Get details about si/shRNA Gene Silencers

See Protocol Online.

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Beskrivning:

20 µl of each primer set is provided at a concentration of 10 µM
FAT1 Primer (m) includes A set (forward & reverse) and B set (forward & reverse) primers
Primer sets are recommended for semi-quantitative nested reverse transcriptase PCR

See Protocol Online.

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Beskrivning:

20 µg, up to 20 transfections
shRNA Plasmids are provided as a pool of three to five lentiviral vector plasmids which each encode a target specific 19-25 nt shRNA with a 6 bp loop
provided as transfection-ready purified plasmid DNA
After transfection, cells stably expressing shRNA can be selected by puromycin treatment

See Protocol Online.

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Beskrivning:

200 µl viral stock containing 10⁶ infectious lentiviral transducing particles per ml, sufficient for 10-20 transductions
Lentiviral Particles are provided as transduction-ready viruses for targeted gene silencing in mammalian cells (human or mouse)
The Lentiviral Particles generally contain three to five expression constructs, each construct encoding a target specific 19-25 nt shRNA with a 6 bp loop
After transduction, cells stably expressing shRNA can be selected by puromycin treatment
Lentiviral particles can be employed in standard Biosafety Level 2 tissue culture facilities (and should be treated with the same level of caution as with any other potentially infectious reagent). Lentiviral particles are replication-incompetent and are designed to self-inactivate after transduction and integration of shRNA constructs into genomic DNA of target cells.

See Protocol Online.

DOWNLOADS

QUICK LINKS