Anti-Cdt1 Antibody (F-6) is a mouse monoclonal IgG2b κ Cdt1 antibody, cited in 13 publications, provided at 200 µg/ml
raised against amino acids 247-546 mapping at the C-terminus of Cdt1 of human origin
Anti-Cdt1 Antibody (F-6) is recommended for detection of Cdt1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
Anti-Cdt1 Antibody (F-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Cdt1 (F-6): sc-365305.
m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Cdt1 Antibody (F-6) for WB and IHC(P) applications. These reagents are now offered in bundles with Cdt1 Antibody (F-6) (see ordering information below).
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Cdt1 Antibody (F-6) is a high quality monoclonal Cdt1 antibody (also designated CDT1 antibody or DUP antibody) suitable for the detection of the Cdt1 protein of mouse, rat and human origin. Cdt1 Antibody (F-6) is available as both the non-conjugated anti-Cdt1 antibody form, as well as multiple conjugated forms of anti-Cdt1 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Human Cdt1 is a nuclear localizing replication initiation factor that is expressed only during the G1 and S phases of the cell cycle. In conjunction with Cdc18, Cdt1 is required to load the MCM protein Cdc21 onto chromatin at the end of mitosis which is necessary to initiate DNA replication. After S-phase onset, Cdt1 protein levels decrease and are barely detectable in cells in early S-phase or G2. However, Cdt1 mRNA is expressed in S-phase-arrested cells, and its levels do not change dramatically during the cell cycle, suggesting that proteolytic degradation rather than transcriptional controls ensure proper accumulation of Cdt1. Cdt1 can associate with the DNA replication inhibitor geminin, which is present in the S and G2 phases of the cell cycle. Inhibition of DNA replication by geminin in cell-free DNA replication extracts can be reversed by the addition of excess Cdt1. Geminin may be responsible for preventing inappropriate origin firing by targeting Cdt1.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
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To place an order using RMB or to ship to mainland China, please visit www.scbio.cn