Anti-CD3-ζ Antibody (6B10.2) is a mouse monoclonal IgG1 κ CD3-ζ antibody, cited in 248 publications, provided at 200 µg/ml
raised against amino acids 36-54 mapping within an N-terminal domain of CD3-ζ of human origin
Anti-CD3-zeta Antibody (6B10.2) is recommended for detection of CD3-ζ of mouse, rat and human origin by WB, IP, IF, IHC(P) and FCM
Anti-CD3-zeta Antibody (6B10.2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to either PerCP, PerCP-Cy5.5 or Alexa Fluor® 405 for IF, IHC(P) and FCM
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CD3-ζ (6B10.2): sc-1239.
m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CD3-ζ Antibody (6B10.2) for WB and IHC(P) applications. These reagents are now offered in bundles with CD3-ζ Antibody (6B10.2) (see ordering information below).
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
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Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
CD3-ζ Antibody (6B10.2) is a high quality monoclonal CD3-zeta antibody (also designated CD247 antibody, or CD3 zeta antibody) suitable for the detection of the CD3-zeta protein of mouse, rat and human origin. CD3-ζ Antibody (6B10.2) is available as both the non-conjugated anti-CD3-zeta antibody form, as well as multiple conjugated forms of anti-CD3-zeta antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. The T cell antigen receptor (TCR) recognizes foreign antigens and translates such recognition events into intracellular signals that elicit a change in the cell from a dormant to an activated state. Much of this signaling process can be attributed to a multisubunit complex of proteins that associates directly with the TCR. This complex has been designated CD3 (cluster of differentiation 3). It is composed of five invariant polypeptide chains that associate to form three dimers: a heterodimer of gamma and epsilon chains (γε), a heterodimer of delta and epsilon chains (δε) and a homodimer of two zeta chains (ζζ) or a heterodimer of zeta and eta chains (ζη). The ζ and η chains are encoded by the same gene but differ in their carboxyl-terminal ends due to an alternative splicing event. The γ, ε and δ chains each contain a single copy of a conserved immunoreceptor tyrosine-based activation motif (ITAM). In contrast, the ζ chain contains three consecutive copies of the same motif. Phosphorylated ITAMs act as docking sites for protein kinases such as ZAP-70 and Syk and are also capable of regulating their kinase activity. The crystal structure of the ZAP-70 SH2 domains bound to the ζ chain ITAMs has been solved.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
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