caspase-3 p11 Antibody (C-6) is a mouse monoclonal IgG1 λ, cited in 115 publications, provided at 200 µg/ml
specific for an epitope mapping between amino acids 185-211 at the N-terminus of caspase-3 p11 of human origin
Anti-caspase-3 p11 Antibody (C-6) is recommended for detection of caspase-3 p11 subunit and full length procaspase-3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
Anti-caspase-3 p11 Antibody (C-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of caspase-3 p11 (C-6): sc-271759.
m-IgGλ BP-HRP (mouse IgGλ binding protein-HRP) is the preferred secondary detection reagent for caspase-3 p11 Antibody (C-6) for WB and IHC(P) applications. This reagent is now offered in a bundle with caspase-3 p11 Antibody (C-6) (see ordering information below). For additional m-IgGλ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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caspase-3 p11 Antibody (C-6) is an IgG1 λ mouse monoclonal caspase-3 p11 antibody suitable for the detection of caspase-3 p11 subunit and full length procaspase-3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. caspase-3 p11 Antibody (C-6) is available as the non-conjugated anti-caspase-3 p11 antibody form, as well as multiple conjugated forms of anti-caspase-3 p11 antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Caspase-3, also known as apopain, SCA-1, Yama and CPP32, is an aspartate-specific cysteine protease that belongs to the ICE subfamily of caspases. Caspase-3 is expressed in cells as an inactive precursor from which the p17 and p11 subunits of the mature caspase-3 are proteolytically generated during apoptosis. The caspase-3 precursor is first cleaved at Asp 175-Ser 176 to produce the p11 subunit and the p20 peptide. Subsequently, the p20 peptide is cleaved at Asp 28-Ser 29 to generate the mature p17 subunit. The active caspase-3 enzyme is a heterodimer composed of two p17 and two p11 subunits. At the onset of apoptosis, caspase-3 proteolytically cleaves PARP at a Asp 216-Gly 217 bond. During the execution of the apoptotic cascade, activated caspase-3 releases SREBP from the membrane of the ER in a proteolytic reaction that is distinct from their normal sterol-dependent activation. Caspase-3 cleaves and activates SREBPs between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase-3 also cleaves and activates caspase-6, -7 and -9. The human caspase-3 gene encodes a cytoplasmic protein that is highly expressed in lung, spleen, heart, liver, kidney and cells of the immune system.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz® conjugates which eliminates the need for a secondary antibody.
Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-caspase-3 p11 Antibody (C-6) has 115 citations in a variety of scientific publications.
Primary antibodies like Anti-caspase-3 p11 Antibody (C-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.
caspase-3 p11 Antibody (C-6) Specifications
Alternate Product Name
Product Clone Name
IgG1 lambda light chain
caspase-3 p11 subunit and full length procaspase-3
mouse, rat, human
Additional Mammalian Reactivity
Additional Non-Mammalian Reactivity
WB, IP, IF, IHC(P), ELISA
Each vial contains 200 µg IgG1 lambda light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin
115 citations in various scientific publications
Recommended Secondary Detection Reagents
Mouse IgG Lambda Light Chain Binding Proteins
m-IgGλ BP-HRP: sc-516132 (Mouse IgGλ binding protein conjugated to horseradish peroxidase)
m-IgGλ BP-B: sc-516197 (Mouse IgGλ binding protein conjugated to biotin)
m-IgGλ BP-FITC: sc-516185 (Mouse IgGλ light chain binding protein conjugated to fluorescein isothiocyanate)
m-IgGλ BP-PE: sc-516186 (Mouse IgGλ light chain binding protein conjugated to phycoerythrin)
m-IgGλ BP-CFL 488: sc-516190 (Mouse IgGλ binding protein conjugated to CruzFluor™ 488)
m-IgGλ BP-CFL 555: sc-516191 (Mouse IgGλ binding protein conjugated to CruzFluor™ 555)
m-IgGλ BP-CFL 594: sc-516192 (Mouse IgGλ binding protein conjugated to CruzFluor™ 594)
m-IgGλ BP-CFL 647: sc-516193 (Mouse IgGλ binding protein conjugated to CruzFluor™ 647)
m-IgGλ BP-CFL 680: sc-516194 (Mouse IgGλ binding protein conjugated to CruzFluor™ 680)
m-IgGλ BP-CFL 790: sc-516195 (Mouse IgGλ binding protein conjugated to CruzFluor™ 790)
Primary antibodies for non-mammalian targets are useful in detecting proteins of plant, viral, bacterial, avian, fish, yeast and invertebrates. Some examples include Drosophila, zebrafish, Xenopus, Arabidopsis, C. elegans, Clostridium, Campylobacter, Heliobacter pylori, Hepatitis, HIV, HPV, HSV, Influenza, Klebsiella, M. tuberculosis, Plasmodium, Pseudomonas, RSV, Saccharomyces cerevisiae, Salmonella, Schistosoma, Shigella and Staphylococcus.
Santa Cruz Biotechnology monoclonal antibodies can be used in assays including flow cytometry, immunoprecipitation, Western blot analysis, and immunostaining. Anti-caspase-3 p11 Antibody (C-6) is recommended for use in the following applications: WB (Western blotting), IP (Immunoprecipitation), IF (Immunofluorescence), IHC(P) (Immunohistochemistry - Paraffin Embedded), ELISA (Enzyme-Linked Immunosorbent Assay).
Mouse secondary antibodies, control sera and control immunoglobulins are also offered to be used in combination with our primary monoclonal antibodies.
Tissue extracts, nuclear extracts and whole cell lysates are used with Santa Cruz Biotechnology monoclonal antibodies for western blotting or immunoprecipitation positive controls. Nuclear extracts are ideal for western blot analysis or gel shift assays positive controls. Phospho-enriched whole cell lysates and tissue extracts are available for phospho-specific antibody positive controls.
Monoclonal secondary antibodies are offered in various conjugated forms, including: Biotin (B), CruzFluor™ 790, 680, 647, 594, 555, and 488, FITC, HRP, PerCP, PerCP-Cy5.5, Texas Red (TR), and TRITC (tetramethyl rhodamine isothiocyanate) (R), for research applications like Flow Cytometry (FCM), Immunofluorescence (IF), Immunohistochemistry (IHC) staining and Western Blotting (WB). Not intended for diagnostic or therapeutic use.
Affinity purified immunoglobulins (IgGs)—conjugated and unconjugated—are available as negative controls for flow cytometry (FCM), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP) and Western Blotting (WB) applications.
Normal sera for immunohistochemistry (IHC) applications are available as neat serum for blocking reagents. The serum which is used should match the species of the secondary antibody.
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