caspase-3 Antibody (31A1067): sc-56053

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- caspase-3 Antibody (31A1067) is a mouse monoclonal IgG₁, cited in 221 publications, provided at 50 µg/0.5 ml
- raised against full length caspase-3 of human origin
- recommended for detection of caspase-3 and full length procaspase-3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- See caspase-3 p11 (C-6): sc-271759 for caspase-3 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor^® 488, 594, 647, 680 and 790.
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of caspase-3 (31A1067): sc-56053.
- m-IgG Fc BP-HRP and m-IgG₁ BP-HRP are the preferred secondary detection reagents for caspase-3 Antibody (31A1067) for WB and IHC(P) applications. These reagents are now offered in bundles with caspase-3 Antibody (31A1067) (see ordering information below).

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caspase-3 Antibody (31A1067) is an IgG1 mouse monoclonal caspase-3 antibody suitable for the detection of caspase-3 and full length procaspase-3 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. caspase-3 Antibody (31A1067) is available as the non-conjugated anti-caspase-3 antibody form. Caspase-3, also known as apopain, SCA-1, Yama and CPP32, is an aspartate-specific cysteine protease that belongs to the ICE subfamily of caspases. Caspase-3 is expressed in cells as an inactive precursor from which the p17 and p11 subunits of the mature caspase-3 are proteolytically generated during apoptosis. The caspase-3 precursor is first cleaved at Asp175-Ser176 to produce the p11 subunit and the p20 peptide. Subsequently, the p20 peptide is cleaved at Asp28-Ser29 to generate the mature p17 subunit. The active caspase-3 enzyme is a heterodimer composed of two p17 and two p11 subunits. At the onset of apoptosis, caspase-3 proteolytically cleaves PARP at an Asp216-Gly217 bond. During the execution of the apoptotic cascade, activated caspase-3 releases SREBP from the membrane of the ER in a proteolytic reaction that is distinct from their normal sterol-dependent activation. Caspase-3 cleaves and activates SREBPs between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase-3 also cleaves and activates caspase-6, -7 and -9. The human caspase-3 gene encodes a cytoplasmic protein that is highly expressed in lung, spleen, heart, liver, kidney and cells of the immune system.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Species	Gene Name	Gene ID	Chromosome Location	Isoform (mRNA) Accession Number	Protein Accession Number	OMIM™ Number
Human	CASP3	Entrez Gene ID: 836	4q35.1	XM_011532301, NM_004346, NM_032991	UniProt ID: P42574	600636

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Protocols Online

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-caspase-3 Antibody (31A1067) has 221 citations in a variety of scientific publications.

Primary antibodies like Anti-caspase-3 Antibody (31A1067) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

caspase-3 Antibody (31A1067) Specifications

Alternate Product Name

caspase-3

Product Clone Name

31A1067

Classification

Primary Antibody

Clonality

Monoclonal Antibody

Antibody Isotype

IgG₁

Amino Acids

Epitope Target

caspase-3

Epitope Species

human

Product Reactivity/Detection

caspase-3 and full length procaspase-3

Species Reactivity/Detection

mouse, rat, human

Additional Mammalian Reactivity

N/A

Additional Non-Mammalian Reactivity

N/A

Applications

WB, IP, IF, IHC(P), ELISA

Vialing Information

Each vial contains 50 µg IgG₁ in 0.5 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations

221 citations in various scientific publications

Recommended Secondary Detection Reagents

Mouse IgG Fc Binding Proteins

m-IgG Fc BP-HRP: sc-525409 (Mouse IgG Fc binding protein conjugated to horseradish peroxidase)
m-IgG Fc BP-B: sc-533652 (Mouse IgG Fc binding protein conjugated to biotin)
m-IgG Fc BP-FITC: sc-533651 (Mouse IgG Fc binding protein conjugated to fluorescein isothiocyanate)
m-IgG Fc BP-CFL 488: sc-533653 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 488)
m-IgG Fc BP-CFL 555: sc-533654 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 555)
m-IgG Fc BP-CFL 594: sc-533655 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 594)
m-IgG Fc BP-CFL 647: sc-533656 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 647)
m-IgG Fc BP-CFL 680: sc-533657 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 680)
m-IgG Fc BP-CFL 790: sc-533658 (Mouse IgG Fc binding protein conjugated to CruzFluor™ 790)

Mouse IgG₁ Binding Proteins

m-IgG₁ BP-HRP: sc-525408 (Mouse IgG₁ binding protein conjugated to horseradish peroxidase)
m-IgG₁ BP-B: sc-533660 (Mouse IgG₁ binding protein conjugated to biotin)
m-IgG₁ BP-FITC: sc-533659 (Mouse IgG₁ binding protein conjugated to fluorescein isothiocyanate)
m-IgG₁ BP-CFL 488: sc-533661 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 488)
m-IgG₁ BP-CFL 555: sc-533662 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 555)
m-IgG₁ BP-CFL 594: sc-533663 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 594)
m-IgG₁ BP-CFL 647: sc-533664 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 647)
m-IgG₁ BP-CFL 680: sc-533665 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 680)
m-IgG₁ BP-CFL 790: sc-533666 (Mouse IgG₁ binding protein conjugated to CruzFluor™ 790)

Available Conjugates/Forms

N/A

Primary antibodies for non-mammalian targets are useful in detecting proteins of plant, viral, bacterial, avian, fish, yeast and invertebrates. Some examples include Drosophila, zebrafish, Xenopus, Arabidopsis, C. elegans, Clostridium, Campylobacter, Heliobacter pylori, Hepatitis, HIV, HPV, HSV, Influenza, Klebsiella, M. tuberculosis, Plasmodium, Pseudomonas, RSV, Saccharomyces cerevisiae, Salmonella, Schistosoma, Shigella and Staphylococcus.

Santa Cruz Biotechnology monoclonal antibodies can be used in assays including flow cytometry, immunoprecipitation, Western blot analysis, and immunostaining. Anti-caspase-3 Antibody (31A1067) is recommended for use in the following applications: WB (Western blotting), IP (Immunoprecipitation), IF (Immunofluorescence), IHC(P) (Immunohistochemistry - Paraffin Embedded), ELISA (Enzyme-Linked Immunosorbent Assay).

Mouse secondary antibodies, control sera and control immunoglobulins are also offered to be used in combination with our primary monoclonal antibodies.

Tissue extracts, nuclear extracts and whole cell lysates are used with Santa Cruz Biotechnology monoclonal antibodies for western blotting or immunoprecipitation positive controls. Nuclear extracts are ideal for western blot analysis or gel shift assays positive controls. Phospho-enriched whole cell lysates and tissue extracts are available for phospho-specific antibody positive controls.

Monoclonal secondary antibodies are offered in various conjugated forms, including: Biotin (B), CruzFluor™ 790, 680, 647, 594, 555, and 488, FITC, HRP, PerCP, PerCP-Cy5.5, Texas Red (TR), and TRITC (tetramethyl rhodamine isothiocyanate) (R), for research applications like Flow Cytometry (FCM), Immunofluorescence (IF), Immunohistochemistry (IHC) staining and Western Blotting (WB). Not intended for diagnostic or therapeutic use.

Affinity purified immunoglobulins (IgGs)—conjugated and unconjugated—are available as negative controls for flow cytometry (FCM), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP) and Western Blotting (WB) applications.

Normal sera for immunohistochemistry (IHC) applications are available as neat serum for blocking reagents. The serum which is used should match the species of the secondary antibody.

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