Anti-A-Raf Antibody (A-5) is a mouse monoclonal IgG1 κ A-Raf antibody, cited in 9 publications, provided at 200 µg/ml
raised against amino acids 141-210 mapping within an internal region of A-Raf of human origin
Anti-A-Raf Antibody (A-5) is recommended for detection of A-Raf of mouse, rat and human origin by WB, IP, IF and ELISA
Anti-A-Raf Antibody (A-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of A-Raf (A-5): sc-166771.
m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for A-Raf Antibody (A-5) for WB applications. These reagents are now offered in bundles with A-Raf Antibody (A-5) (see ordering information below).
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
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SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
A-Raf Antibody (A-5) is a high quality monoclonal A-Raf antibody (also designated A-Raf Proto-Oncogene Serine/Threonine Kinase antibody, Serine/Threonine-Protein Kinase A-Raf antibody, Proto-Oncogene A-Raf-1 antibody, Proto-Oncogene Pks antibody, ARAF1 antibody, PKS2 antibody, A-Raf Proto-Oncogene Serine/Threonine-Protein Kinase antibody, Ras-Binding Protein DA-Raf antibody, Proto-Oncogene A-Raf antibody, Oncogene ARAF1 antibody, A-RAF antibody, or RAFA1 antibody) suitable for the detection of the A-Raf protein of mouse, rat and human origin. A-Raf Antibody (A-5) is available as both the non-conjugated anti-A-Raf antibody form, as well as multiple conjugated forms of anti-A-Raf antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/ threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. A-Raf, a second member of the Raf gene family of serine/threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of A-Raf is found at highest levels in urogenital tissues and kidney and at lowest level in brain tissue.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
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