Z-VAD(OMe)-FMK is an irreversible and cell permeable broad-spectrum Caspase Inhibitor. It has been shown to inhibit Fas-mediated apoptosis in Jurkat T cells and apoptosis in THP.1 cells induced by diverse stimuli (including cycloheximide, thapsigargin, etoposide and staurosporine). Z-VAD(OMe)-FMK is an inhibitor of caspase-1 and caspase-3.
1. Chow, S.C., et al. 1995. FEBS Lett. 364: 134-138. PMID: 7538469 2. Zhu, H., et al. 1995. FEBS Lett. 374: 303-308. PMID: 7589559 3. Sarin, A., et al. 1996. J. Exp. Med. 184: 2445-2450. PMID: 8976202 4. Slee, E.A., et al. 1996. Biochem. J. 315: 21-24. PMID: 8670109 5. Shao, R.G., et al. 1997. J. Biol. Chem. 272: 31321-31325. PMID: 9395460 6. Chandler, J.M., et al. 1998. J. Biol. Chem. 273, 10815-10818. PMID: 9556551 7. Farber, A., et al. 1999. J. Vasc. Surg. 30: 752-760. PMID: 10514215 8. Mundle, S.D., et al.1999. Cancer Lett. 140: 201-207. PMID: 10403560 9. Piguet, P.F., et al. 1999. Lab. Invest. 79: 495-500. PMID: 10212002 10. Warny, M. and Kelly, C.P. 1999. Am. J. Physiol. 276: C717-C724. PMID: 10070000 11. Shimohama, S. 2000. Apoptosis 5: 9-16. PMID: 1122749 12. Dong, Z., et al. 2000. Biochem. J. 347: 669-677. PMID: 10769169 13. Wiessner, C., et al. 2000. Cell Mol. Biol. 46: 53-62. PMID: 10726971 14. Barsoum, M.J., et al. Embo J. 25: 3900-3911. PMID: 16874299 15. Lokshin, A.E. et al. 2002. Int. Immunol. 14(9): 1027-1037. PMID: 12202400
Soluble in DMSO (20 mg/ml), DMF (7 mg/ml), ethanol (1.3 mg/ml), and methanol:chloroform (1:1) (10 mg/ml). Insoluble in water.
Store at -20° C
267.59° C (Predicted)
732.37° C at 760 mmHg (Predicted)
1.21 g/cm3 (Predicted)
n20D 1.51 (Predicted)
pKa: 11.05 (Predicted)
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
I need to get 600mg of Z-VAD(OMe)-FMK [sc-311561]. Please provide me with a quote for this purchase at your earliest continent. Also, CHOP is nonprofit hospital. Will be get any discount because of this? Regards
Asked by: CHOP
Thank you for your question. The product is currently in stock. We offer over night shipment to many locations. Please contact our Customer Service department to place a product order or to ask for a sales quotation inlcuding delivery time.
Answered by: Technical Support
Date published: 2018-11-26
What is the half life of this product if it is added into the culture medium?
Asked by: two2igm05
The half-life of this product in vitro is ≤40 minutes.
Answered by: Chemical Support 4
Date published: 2017-02-25
How do I use Z-VAD(OMe)-FMK to inhibit caspase-3/7?
Asked by: SCM4
The publication by Fox, et al. (PMID: 26771236) describes the use of “z-VAD” to inhibit caspase-3/7.
Rated 5 out of
Good prduct, good technical serviceI made a purchase of z-VAD(OMe)-FMK to replicate previous findings in other AML cell lines. The current line under investigation undergoes apoptosis in response to a test compound with slower kinetics than other lines with which I had already worked. Still, I was able to show inhibition of apoptosis with 50 and 100 uM z-VAD out to 16 hours with partial effect of 50 uM at 24 hours. It seems that the higher dose is required for later time points in my AML cell lines. (I had previously seen nearly complete reversal of cell death at 10 hours using 50 uM z-VAD in other lines).
Additionally, I was able to easily dissolve the compound at 100mM stock solution and it survived two freeze-thaw cycles and retained its activity.
Overall, a good product.
In addition, when an overnight shipment failed to arrive on a Friday due to weather and sat in a warehouse at RT over the weekend, the technical service worked with me and eventually sent a new vial for me to test. In the end, the original vial showed activity that was only slightly reduced compared to the new vial that did not have any extended time out of the freezer (as powder).
Date published: 2018-02-06
Rated 5 out of
the advantages of itit is an irreversible broad-spectrum caspase inhibitor of permeable cells, which can block apoptosis, and the effects of it is good ,but which may be harmful to the environment.
Date published: 2017-01-08
Rated 5 out of
BergerBerger, AK. et al. (PubMed 23674612) used product sc-311561 (Z-VAD(OMe)-FMK), a cell permeable caspase inhibitor, to determine if reovirus induces caspase-independent cell death. -SCBT Publication Review
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