Z-VAD(OMe)-FMKAn irreversible and cell permeable broad-spectrum Caspase Inhibitor

Z-VAD(OMe)-FMK (CAS 187389-52-2)

Z-VAD(OMe)-FMK | CAS 187389-52-2 is rated 5.0 out of 5 by 3.
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Synonym: Z-VAD-FMK; pan-Caspase Inhibitor
Application: An irreversible and cell permeable broad-spectrum Caspase Inhibitor
CAS Number: 187389-52-2
Purity: ≥95%
Molecular Weight: 467.49
Molecular Formula: C22H30FN3O7
* Refer to Certificate of Analysis for lot specific data (including water content).
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Z-VAD(OMe)-FMK is an irreversible and cell permeable broad-spectrum Caspase Inhibitor displaying an IC50 of 5.8 nM in HepG2 cells. It has been shown to inhibit caspase-1 and caspase-3. It has also been shown to inhibit Fas-mediated apoptosis in Jurkat T cells and apoptosis in THP.1 cells induced by diverse stimuli (including cycloheximide, thapsigargin, etoposide and staurosporine). Furthermore, anti-CD95-induced hepatocyte apoptosis was prevented in a concentration-dependent manner by inhibitor Z-VAD-FMK (IC50 = 1.5 nM). Additionally, it has been shown that pretreatment of MDA-MB-231 and HL-60 cells with Z-VAD(OMe)-FMK also inhibits apoptosis.


References

1. Chow, S.C., et al. 1995. FEBS Lett. 364: 134-138. PMID: 7538469

2. Zhu, H., et al. 1995. FEBS Lett. 374: 303-308. PMID: 7589559

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4. Slee, E.A., et al. 1996. Biochem. J. 315: 21-24. PMID: 8670109

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9. Mundle, S.D., et al.1999. Cancer Lett. 140: 201-207. PMID: 10403560

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11. Warny, M. and Kelly, C.P. 1999. Am. J. Physiol. 276: C717-C724. PMID: 10070000

12. Shimohama, S. 2000. Apoptosis 5: 9-16. PMID: 1122749

13. Dong, Z., et al. 2000. Biochem. J. 347: 669-677. PMID: 10769169

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15. Lokshin, A.E. et al. 2002. Int. Immunol. 14(9): 1027-1037. PMID: 12202400

16. Barsoum, M.J., et al. 2006. Embo J. 25: 3900-3911. PMID: 16874299

17. de Souza Oliveria, M., et al. 2018. Int J Mol Sci. 19(6): 1609. PMID: 29848969

18. Hwang, E., et al. 2018. Ann Surg Treat Res. 95(5): 240–248. PMID: 30402442

Usage :
Stock solutions should be divided into aliquots and stored at -20°C for up to 1 month.
Physical State :
Solid
Sequence :
Z-Val-Ala-Asp(OMe)-fluoromethylketone
Solubility :
Soluble in DMSO (20 mg/ml), DMF (7 mg/ml), ethanol (1.3 mg/ml), and methanol:chloroform (1:1) (10 mg/ml). Insoluble in water.
Storage :
Store at -20° C
Melting Point :
267.59° C (Predicted)
Boiling Point :
732.37° C at 760 mmHg (Predicted)
Density :
1.21 g/cm3 (Predicted)
Refractive Index :
n20D 1.51 (Predicted)
pK Values :
pKa: 11.05 (Predicted)
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
PubChem CID :
5497174
MDL Number :
MFCD02684037
SMILES :
C[C@@H](C(=O)N[C@@H](CC(=O)OC)C(=O)CF)NC(=O)[C@H](C(C)C)NC(=O)OCC1=CC=CC=C1

Download SDS (MSDS)

Certificate of Analysis

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Z-VAD(OMe)-FMK  Product Citations

See how others have used Z-VAD(OMe)-FMK. Click on the entry to view the PubMed entry .

Citations 1 to 10 of 86 total

PMID: # 30692621  Choi, YJ. et al. 2019. Nat. Immunol. 20: 276-287.

PMID: # 30795621  Nováková, J.|Talacko, P.|Novák, P.|Vališ, K.| et al. 2019. Cells. 8:

PMID: # 31262874  Seo, YS. et al. 2019. Anticancer Res. 39: 3499-3506.

PMID: # 30914761  Yu, P. et al. 2019. Acta Pharmacol. Sin.

PMID: # 30967464  Giebel, AM.|Hu, S.|Rajaram, K.|Finethy, R.|Toh, E.|Brothwell, JA.|Morrison, SG.|Suchland, RJ.|Stein, BD.|Coers, J.|Morrison, RP.|Nelson, DE.| et al. 2019. MBio. 10:

PMID: # 31031142  McNamara, DE.|Dovey, CM.|Hale, AT.|Quarato, G.|Grace, CR.|Guibao, CD.|Diep, J.|Nourse, A.|Cai, CR.|Wu, H.|Kalathur, RC.|Green, DR.|York, JD.|Carette, JE.|Moldoveanu, T.| et al. 2019. Cell Chem Biol. 26: 863-877.e7.

PMID: # 30603830  Valli, F. et al. 2019. Apoptosis. 24: 119-134.

PMID: # 30550847  Wu, X. et al. 2019. Microb. Pathog. 127: 277-287.

PMID: # 30918505  Fernandes, MHV.|Maggioli, MF.|Otta, J.|Joshi, LR.|Lawson, S.|Diel, DG.| et al. 2019. Front Immunol. 10: 363.

PMID: # 29317521  Lee, HY. et al. 2018. Sci Signal. 11:

Citations 1 to 10 of 86 total

I need to get 600mg of Z-VAD(OMe)-FMK [sc-311561]. Please provide me with a quote for this purchase at your earliest continent. Also, CHOP is nonprofit hospital. Will be get any discount because of this? Regards

Asked by: CHOP
Thank you for your question. The product is currently in stock. We offer over night shipment to many locations. Please contact our Customer Service department to place a product order or to ask for a sales quotation inlcuding delivery time.
Answered by: Technical Support
Date published: 2018-11-26

What is the half life of this product if it is added into the culture medium?

Asked by: two2igm05
The half-life of this product in vitro is ≤40 minutes.
Answered by: Chemical Support 4
Date published: 2017-02-25

How do I use Z-VAD(OMe)-FMK to inhibit caspase-3/7? 

Asked by: SCM4
The publication by Fox, et al. (PMID: 26771236) describes the use of “z-VAD” to inhibit caspase-3/7.
Answered by: Tech Service 11
Date published: 2017-02-24
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Rated 5 out of 5 by from Good prduct, good technical service I made a purchase of z-VAD(OMe)-FMK to replicate previous findings in other AML cell lines. The current line under investigation undergoes apoptosis in response to a test compound with slower kinetics than other lines with which I had already worked. Still, I was able to show inhibition of apoptosis with 50 and 100 uM z-VAD out to 16 hours with partial effect of 50 uM at 24 hours. It seems that the higher dose is required for later time points in my AML cell lines. (I had previously seen nearly complete reversal of cell death at 10 hours using 50 uM z-VAD in other lines). Additionally, I was able to easily dissolve the compound at 100mM stock solution and it survived two freeze-thaw cycles and retained its activity. Overall, a good product. In addition, when an overnight shipment failed to arrive on a Friday due to weather and sat in a warehouse at RT over the weekend, the technical service worked with me and eventually sent a new vial for me to test. In the end, the original vial showed activity that was only slightly reduced compared to the new vial that did not have any extended time out of the freezer (as powder).
Date published: 2018-02-06
Rated 5 out of 5 by from the advantages of it it is an irreversible broad-spectrum caspase inhibitor of permeable cells, which can block apoptosis, and the effects of it is good ,but which may be harmful to the environment.
Date published: 2017-01-08
Rated 5 out of 5 by from Berger Berger, AK. et al. (PubMed 23674612) used product sc-311561 (Z-VAD(OMe)-FMK), a cell permeable caspase inhibitor, to determine if reovirus induces caspase-independent cell death. -SCBT Publication Review
Date published: 2015-07-10
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