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Z-VAD(OMe)-FMK (CAS 187389-52-2)

Z-VAD(OMe)-FMK | CAS 187389-52-2 is rated 5.0 out of 5 by 3.

| See product citations (59)

Synonym: Z-VAD-FMK; pan-Caspase Inhibitor

Application: An irreversible and cell permeable broad-spectrum Caspase Inhibitor

CAS Number: 187389-52-2

Purity: ≥95%

Molecular Weight: 467.49

Molecular Formula: C₂₂H₃₀FN₃O₇

* Refer to Certificate of Analysis for lot specific data (including water content).

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Ordering Information

PRODUCT NAME	CATALOG #	UNIT	PRICE	QTY		FAVORITES
Z-VAD(OMe)-FMK	sc-311561	1 mg	$128.00
Z-VAD(OMe)-FMK	sc-311561A	5 mg	$500.00

Z-VAD(OMe)-FMK is an irreversible and cell permeable broad-spectrum Caspase Inhibitor. It has been shown to inhibit Fas-mediated apoptosis in Jurkat T cells and apoptosis in THP.1 cells induced by diverse stimuli (including cycloheximide, thapsigargin, etoposide and staurosporine). Z-VAD(OMe)-FMK is an inhibitor of caspase-1 and caspase-3.

References

1. Chow, S.C., et al. 1995. FEBS Lett. 364: 134-138. PMID: 7538469
2. Zhu, H., et al. 1995. FEBS Lett. 374: 303-308. PMID: 7589559
3. Sarin, A., et al. 1996. J. Exp. Med. 184: 2445-2450. PMID: 8976202
4. Slee, E.A., et al. 1996. Biochem. J. 315: 21-24. PMID: 8670109
5. Shao, R.G., et al. 1997. J. Biol. Chem. 272: 31321-31325. PMID: 9395460
6. Chandler, J.M., et al. 1998. J. Biol. Chem. 273, 10815-10818. PMID: 9556551
7. Farber, A., et al. 1999. J. Vasc. Surg. 30: 752-760. PMID: 10514215
8. Mundle, S.D., et al.1999. Cancer Lett. 140: 201-207. PMID: 10403560
9. Piguet, P.F., et al. 1999. Lab. Invest. 79: 495-500. PMID: 10212002
10. Warny, M. and Kelly, C.P. 1999. Am. J. Physiol. 276: C717-C724. PMID: 10070000
11. Shimohama, S. 2000. Apoptosis 5: 9-16. PMID: 1122749
12. Dong, Z., et al. 2000. Biochem. J. 347: 669-677. PMID: 10769169
13. Wiessner, C., et al. 2000. Cell Mol. Biol. 46: 53-62. PMID: 10726971
14. Barsoum, M.J., et al. Embo J. 25: 3900-3911. PMID: 16874299
15. Lokshin, A.E. et al. 2002. Int. Immunol. 14(9): 1027-1037. PMID: 12202400

Physical State :

Solid

Sequence :

Z-Val-Ala-Asp(OMe)-fluoromethylketone

Solubility :

Soluble in DMSO (20 mg/ml), DMF (7 mg/ml), ethanol (1.3 mg/ml), and methanol:chloroform (1:1) (10 mg/ml). Insoluble in water.

Storage :

Store at -20° C

Melting Point :

267.59° C (Predicted)

Boiling Point :

732.37° C at 760 mmHg (Predicted)

Density :

1.21 g/cm³ (Predicted)

Refractive Index :

n²⁰_D 1.51 (Predicted)

pK Values :

pK_a: 11.05 (Predicted)

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

WGK Germany :

PubChem CID :

5497174

MDL Number :

MFCD02684037

SMILES :

C[C@@H](C(=O)N[C@@H](CC(=O)OC)C(=O)CF)NC(=O)[C@H](C(C)C)NC(=O)OCC1=CC=CC=C1

Download SDS (MSDS)

Certificate of Analysis

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Z-VAD(OMe)-FMKProduct Citations

See how others have used Z-VAD(OMe)-FMK. Click on the entry to view the PubMed entry .

Citations 1 to 10 of 59 total

PMID: # 28414095
Waetzig, V. et al. 2017. Crosstalk control and limits of physiological c-Jun N-terminal kinase activity for cell viability and neurite stability in differentiated PC12 cells. Mol. Cell. Neurosci.. 82: 12-22.

PMID: # 28138710
Zhang, L. et al. 2017. Moscatilin induces apoptosis of pancreatic cancer cells via reactive oxygen species and the JNK/SAPK pathway. Mol Med Rep. 15: 1195-1203.

PMID: # 28749466
Xia, D. et al. 2017. Knockout of MARCH2 inhibits the growth of HCT116 colon cancer cells by inducing endoplasmic reticulum stress. Cell Death Dis. 8: e2957.

PMID: # 29074567
Gendarme, M. et al. 2017. Image-based drug screen identifies HDAC inhibitors as novel Golgi disruptors synergizing with JQ1. Mol. Biol. Cell.

PMID: # 27693581
Antunes, F. et al. 2017. Fasting boosts sensitivity of human skin melanoma to cisplatin-induced cell death. Biochem. Biophys. Res. Commun.. 485: 16-22.

PMID: # 29113338
Chen, P. et al. 2017. MiR-23a modulates X-linked inhibitor of apoptosis-mediated autophagy in human luminal breast cancer cell lines. Oncotarget. 8: 80709-80721.

PMID: # 28139733
Liu, L. et al. 2017. Lysosomal dysfunction and autophagy blockade contribute to IMB-6G-induced apoptosis in pancreatic cancer cells. Sci Rep. 7: 41862.

PMID: # 28300624
da Silva, DC. et al. 2017. Neurotoxicity of the steroidal alkaloids tomatine and tomatidine is RIP1 kinase- and caspase-independent and involves the eIF2α branch of the endoplasmic reticulum. J. Steroid Biochem. Mol. Biol.. 171: 178-186.

PMID: # 26901245
Xia, X. et al. 2016. Tetrachlorobenzoquinone Stimulates NLRP3 Inflammasome-Mediated Post-Translational Activation and Secretion of IL-1β in the HUVEC Endothelial Cell Line. Chemical research in toxicology. 29: 421-9.

PMID: # 27055473
Yu, Y. et al. 2016. Abrin P2 suppresses proliferation and induces apoptosis of colon cancer cells via mitochondrial membrane depolarization and caspase activation. Acta Biochim. Biophys. Sin. (Shanghai). 48: 420-9.

Citations 1 to 10 of 59 total

What is the half life of this product if it is added into the culture medium?

Asked by: two2igm05

The half-life of this product in vitro is ≤40 minutes.

Answered by: Chemical Support 4

Date published: 2017-02-25

How do I use Z-VAD(OMe)-FMK to inhibit caspase-3/7? 

Asked by: SCM4

The publication by Fox, et al. (PMID: 26771236) describes the use of “z-VAD” to inhibit caspase-3/7.

Answered by: Tech Service 11

Date published: 2017-02-24

I need to get 600mg of Z-VAD(OMe)-FMK [sc-311561]. Please provide me with a quote for this purchase at your earliest continent. Also, CHOP is nonprofit hospital. Will be get any discount because of this? Regards

Asked by: CHOP

Rated 5 out of 5 by cbr8 from Good prduct, good technical service I made a purchase of z-VAD(OMe)-FMK to replicate previous findings in other AML cell lines. The current line under investigation undergoes apoptosis in response to a test compound with slower kinetics than other lines with which I had already worked. Still, I was able to show inhibition of apoptosis with 50 and 100 uM z-VAD out to 16 hours with partial effect of 50 uM at 24 hours. It seems that the higher dose is required for later time points in my AML cell lines. (I had previously seen nearly complete reversal of cell death at 10 hours using 50 uM z-VAD in other lines). Additionally, I was able to easily dissolve the compound at 100mM stock solution and it survived two freeze-thaw cycles and retained its activity. Overall, a good product. In addition, when an overnight shipment failed to arrive on a Friday due to weather and sat in a warehouse at RT over the weekend, the technical service worked with me and eventually sent a new vial for me to test. In the end, the original vial showed activity that was only slightly reduced compared to the new vial that did not have any extended time out of the freezer (as powder).

Date published: 2018-02-06

Rated 5 out of 5 by jackfeng from the advantages of it it is an irreversible broad-spectrum caspase inhibitor of permeable cells, which can block apoptosis, and the effects of it is good ,but which may be harmful to the environment.

Date published: 2017-01-08

Rated 5 out of 5 by JT from Berger Berger, AK. et al. (PubMed 23674612) used product sc-311561 (Z-VAD(OMe)-FMK), a cell permeable caspase inhibitor, to determine if reovirus induces caspase-independent cell death. -SCBT Publication Review

Date published: 2015-07-10

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