Wortmannin A fungal metabolite which inhibits Pl3-kinase

Wortmannin (CAS 19545-26-7)

Wortmannin | CAS 19545-26-7 is rated 4.3 out of 5 by 3.
  • y_2019, m_5, d_21, h_21
  • bvseo_bulk, prod_bvrr, vn_bulk_2.0.12
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_3
  • loc_en_US, sid_3505, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 81ms
  • REVIEWS, PRODUCT
Synonym: KY 12420; SL-2052
Application: A fungal metabolite which inhibits Pl3-kinase
CAS Number: 19545-26-7
Purity: ≥98%
Molecular Weight: 428.43
Molecular Formula: C23H24O8
Supplemental Information: This is classified as a Dangerous Good for transport and may be subject to additional shipping charges.
* Refer to Certificate of Analysis for lot specific data (including water content).
Submit a review for this product and receive 15 CruzCredits

Wortmannin, a fungal metabolite, is a potent and selective inhibitor of PI 3-kinase. Wortmannin inhibits repair of DNA double-strand breaks in irradiated normal human cells. Wortmannin has an in vitro IC50 of around 5 nM, making it a more potent inhibitor than LY 294002 (sc-201426), another commonly used PI 3-kinase inhibitor. Wortmannin displays a similar potency in vitro for the class I, II, and III PI 3-kinase members, although it can also inhibit other PI 3-kinase-related enzymes such as FRAP (mTOR), DNA-PKCS, some phosphatidylinositol 4-kinases, myosin light chain kinase (MLCK) and mitogen-activated protein kinase (MAPK) at high concentrations. Wortmannin has also been reported to inhibit members of the polo-like kinase family with IC50 in the same range as for PI 3-kinase. The half-life of Wortmannin in tissue culture is about 10 minutes due to the presence of the highly reactive C20 carbon that is also responsible for its ability to covalently inactivate PI 3-kinase. Wortmannin is an inhibitor of Fnk and Plk.


References

1. Nakanishi, S., et al. 1992. J. Biol. Chem. 267: 2157-2163. PMID: 1733924
2. Arcaro, A. and Wymann, M.P. 1993. Biochem. J. 296: 297-301. PMID: 8257416
3. Okada, T., et al. 1994. J. Biol. Chem. 269: 3563-3567. PMID: 8106399
4. Okada, T., et al. 1994. J. Biol. Chem. 269: 3568-3573. PMID: 8106400
5. Ui, M., et al. 1995. Trends Biochem. Sci. 20: 303-307. PMID: 7667888
6. Wymann, M.P., et al. 1996. Mol. Cell. Biol. 16: 1722-1733. PMID: 8657148
7. Liu, Y., et al. 2005. Chem. Biol. 12: 99-107. PMID: 15664519

Appearance :
Powder
Physical State :
Solid
Solubility :
Soluble in DMSO (50 mg/ml), ethanol (25 mg/ml), methanol, 1:8 DMSO:PBS(pH 7.2) (~0.1 mg/ml), and DMF (~14 mg/ml).
Storage :
Store at -20° C
Melting Point :
237.52° C (Predicted)
Boiling Point :
~615.6° C at 760 mmHg (Predicted)
Density :
~1.4 g/cm3 (Predicted)
Refractive Index :
n20D 1.59
IC50 :
PI3K enzymes: IC50 = 1-10 nM; class II PI3Ks: IC50 = 5 nM (Drosophila); class II PI3Ks: IC50 = 50 nM (murine); class II PI3Ks: IC50 = 450 nM (human); polo-like kinase 1: IC50 = 24 nM; polo-like kinase 3: IC50 = 49 nM; MAP kinase activation: IC50 = 200-300 nM ( guinea pig neutrophils); myosin light chain kinase: IC50 = 200 nM; PDGF-mediated Ins(3,4,5)P3 formation: IC50 = 5 nM (fibroblasts)
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
RTECS :
CB9641000
PubChem CID :
312145
Merck Index :
14: 10053
MDL Number :
MFCD00133927
EC Number :
214-538-0
Beilstein Registry :
67676
SMILES :
CC(=O)O[C@@H]1C[C@]2([C@@H](CCC2=O)C3=C1[C@]4([C@H](OC(=O)C5=COC(=C54)C3=O)COC)C)C

Download SDS (MSDS)

Certificate of Analysis

Adobe Acrobat Reader is required to reliably view,
print and comment on PDF documents

Wortmannin  Product Citations

See how others have used Wortmannin. Click on the entry to view the PubMed entry .

Citations 1 to 10 of 49 total

PMID: # 28054347  Cobine, CA. et al. 2017. J. Physiol. (Lond.). 595: 2021-2041.

PMID: # 28792845  Mishra, P. et al. 2017. Autophagy. 13: 1556-1572.

PMID: # 26966187  Muhamed, I. et al. 2016. J. Cell. Sci. 129: 1843-54.

PMID: # 27602962  Chen, JC. et al. 2016. Oncotarget. 7: 70276-70289.

PMID: # 27328819  Chen, X. et al. 2016. Scientific reports. 6: 28423.

PMID: # 27138223  Hossini, AM. et al. 2016. PLoS ONE. 11: e0154770.

PMID: # 26648098  Gao, Y. et al. 2016. International journal of oncology. 48: 281-92.

PMID: # 26189652  Nesbitt, H. et al. 2016. J. Cell. Physiol. 231: 473-82.

PMID: # 27238412  Sattayaprasert, P. et al. 2016. J. Mol. Cell. Cardiol.

PMID: # 27619995  Wu, Y. et al. 2016. J. Immunol.

Citations 1 to 10 of 49 total

Wortmannin is said to be unstable in cell culture media ( t1/2 of 10 mins). How can I make serial dilutions in media and use this to treat cells in culture without losing its activity?

Asked by: Simeon
Thank you for your question. Unfortunately, we are not able to find any recommended cell culture protocol for use of Wortmannin, and we have not used it in our own cell culture labs. We did find a paper that may serve as a useful reference, please see PMID: 14622954. Use of high-performance liquid chromatography to characterize the rapid decomposition of wortmannin in tissue culture media:: J.L. Holleran, et al.; Analysis Biochem. 323, 19 (2003)
Answered by: Technical Support
Date published: 2017-05-17

What is the appearance of the compound?

Asked by: two2igm05
Thank you for your question. Wortmannin, sc-3505, is in white or off-white powder form.
Answered by: Chemical Support 4
Date published: 2017-04-05

What is the stability overtime of this product at -20C?

Asked by: Io5ay
Thank you for your question. At -20 degrees, this chemical should undergo no degradation and be stable for a very long time. Please contact Technical Service if you have further questions concerning this product.
Answered by: Tech Service 9
Date published: 2016-12-15
  • y_2019, m_5, d_21, h_21CST
  • bvseo_bulk, prod_bvqa, vn_bulk_2.0.12
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_3
  • loc_en_US, sid_3505, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 136ms
  • QUESTIONS, PRODUCT
Rated 4 out of 5 by from This is working very well in our experiments This is working very well in our experiments.
Date published: 2015-09-03
Rated 4 out of 5 by from This product works fine This product works fine.
Date published: 2015-03-23
Rated 5 out of 5 by from It has been reported that (PubMed ID 24113173) It has been reported that (PubMed ID 24113173) product sc-3505 was successfully used to sensitize melanoma cells for exposure to TRAIL. When used together, Wortmannin enhanced downstream activation of mitochondrial apoptosis pathways. -SCBT Publication Review
Date published: 2015-03-23
  • y_2019, m_5, d_21, h_21
  • bvseo_bulk, prod_bvrr, vn_bulk_2.0.12
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_3
  • loc_en_US, sid_3505, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 221ms
  • REVIEWS, PRODUCT

Santa Cruz Biotechnology, Inc. is a world leader in the development of products for the biomedical research market. Call us Toll Free at 1-800-457-3801.
Copyright © 2007-2019 Santa Cruz Biotechnology, Inc. All Rights Reserved. "Santa Cruz Biotechnology", and the Santa Cruz Biotechnology, Inc. logo, "Santa Cruz Animal Health", "San Juan Ranch", "Supplement of Champions", the San Juan Ranch logo, "Ultracruz", "Chemcruz", "Immunocruz", "Exactacruz", and "EZ Touch" are registered trademarks of Santa Cruz Biotechnology, Inc.
All trademarks are the property of their respective owners.