Western Blotting Luminol Reagentreagent for enhanced chemiluminescence (ECL) detection of Western blots

Western Blotting Luminol Reagent: sc-2048

Western Blotting Luminol Reagent is rated 4.6 out of 5 by 55.
  • y_2019, m_8, d_20, h_6
  • bvseo_bulk, prod_bvrr, vn_bulk_2.0.13
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_55
  • loc_en_US, sid_2048, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 111ms
  • REVIEWS, PRODUCT
Datasheets
    • Santa Cruz Biotechnology Luminol reagent is a non-radioactive, light-emitting system for detecting proteins on a Western blot membrane. Horseradish peroxidase mediates oxidation of Luminol reagent in the presence of hydrogen peroxide and this reaction produces an iridescent light.
    • Western blotting Luminol reagent is recommended for Western blotting enhanced chemiluminescence applications using Horseradish Peroxidase (HRP) conjugated secondary antibodies.
    • One Western blotting Luminol reagent package contains 125 ml each Solution A and Solution B, which combine to form sufficient chemiluminescence reagent for a 2,000 cm squared membrane area.
    • Extended exposure times (multiple exposures) to film made possible by long-duration signal
    • Western blotting Luminol reagent solutions are stable at room temperature for one hour. Store at 2-8° C for longer periods up to 12 months. Store in the dark.
Submit a review for this product and receive 15 CruzCredits

Same problem here! the protocol is still not available on the website and did not arrive with the luminol! Please send it to me asap!

Asked by: Rina
Thank you for your question. The protocol for Western Blotting Luminol Reagent sc-2048 can be found in the datasheet. The datasheet is available from the product page or under the following link: https://datasheets.scbt.com/sc-2048.pdf
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

Would you please tell me how much time it needs to be in the membrane before imaging? 2 min? 5 min?

Asked by: mnopqr
Thank you for your question. We recommend using 0,125 ml per square centimeter of the membrane and incubating the membtane for 1 minute at room temperature. You can find the datasheet with the procedure for this product by following the link below: https://datasheets.scbt.com/sc-2048.pdf
Answered by: SCBT Heidelberg Support
Date published: 2018-12-29

Is it compatible only with mouse secondary? How about rabbit and other?

Asked by: Sireesh
Than you for your question. Our Western Blotting Luminol Reagent: sc-2048 is compatible with any Horseradish Peroxidase (HRP) conjugated secondary antibodies. The host or target species does not make any difference.
Answered by: Technical Support
Date published: 2018-12-14

The user guide on your website is a safety datasheet.I haven't found other guidelines.So if you're convenient, please send me one.I couldn't find mine anywhere.

Asked by: Huai
thank you very much for bringing this to our attention. We will update the corresponding link on our website shortly. I just sent you the technical datasheet with protocol be email.
Answered by: Technical Support Europe
Date published: 2018-08-28

volevo sapere che sensibilità ha questo ECL..fantogram o pico grazie

Asked by: alessandro
Grazie mille per la sua domanda, contatti pure il nostro supporto tecnico via email o telefono per avere informazioni più specifiche sul prodotto.
Answered by: Technical Support Europe
Date published: 2017-11-22
  • y_2019, m_8, d_20, h_6CST
  • bvseo_bulk, prod_bvqa, vn_bulk_2.0.13
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_5
  • loc_en_US, sid_2048, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 98ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Reagent works very well I compared this luminol reagent to a more expensive luminol reagent and there was no difference. Using this luminol reagent will save you money.
Date published: 2019-05-07
Rated 5 out of 5 by from Effective detection of proteins! The solution is effective in detecting proteins. No problems encountered during use.
Date published: 2019-02-04
Rated 5 out of 5 by from Trusted Western Blotting Luminol Reagent We are using SantaCruz Western Blotting Luminol Reagent for a while and never had any problem. Excellent reagent, very stable at 4C.
Date published: 2018-11-23
Rated 1 out of 5 by from No guidance with product No guidance, No protocol provided either with the product, or online. Yes, mix A and B, but how much onto a membrane, for how long? the simple questions!
Date published: 2018-11-15
Rated 5 out of 5 by from Muy buenos resultados estamos muy contentos con el producto y su precio
Date published: 2018-09-05
Rated 4 out of 5 by from Good product It works good in strong expression proteins such as beta-actin.
Date published: 2018-07-04
Rated 5 out of 5 by from ECL for strong antibodies This ECL has not failed me, it is very good for too strong signals
Date published: 2018-06-29
Rated 4 out of 5 by from Excelente reactivo de western blot Compré este producto para la detección de algunas proteinas de reparación de DNA, y los resultados obtenidos fueron excelentes, incluso con aquellas proteínas de baja expresión
Date published: 2018-06-22
  • y_2019, m_8, d_20, h_6
  • bvseo_bulk, prod_bvrr, vn_bulk_2.0.13
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_55
  • loc_en_US, sid_2048, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 47ms
  • REVIEWS, PRODUCT

Santa Cruz Biotechnology, Inc. is a world leader in the development of products for the biomedical research market. Call us Toll Free at 1-800-457-3801.
Copyright © 2007-2019 Santa Cruz Biotechnology, Inc. All Rights Reserved. "Santa Cruz Biotechnology", and the Santa Cruz Biotechnology, Inc. logo, "Santa Cruz Animal Health", "San Juan Ranch", "Supplement of Champions", the San Juan Ranch logo, "Ultracruz", "Chemcruz", "Immunocruz", "Exactacruz", and "EZ Touch" are registered trademarks of Santa Cruz Biotechnology, Inc.
All trademarks are the property of their respective owners.