Water, DEPC-Treated Ready to use sterile filtered RNase free water for reconstitution of lyophilized products

Water, DEPC-Treated (CAS 7732-18-5)

Water, DEPC-Treated | CAS 7732-18-5 is rated 5.0 out of 5 by 1.
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Application: Ready to use sterile filtered RNase free water for reconstitution of lyophilized products
CAS Number: 7732-18-5
Molecular Weight: 18.02
Molecular Formula: H2O
* Refer to Certificate of Analysis for lot specific data (including water content).
Submit a review for this product and receive 15 CruzCredits

Water, DEPC-Treated is a DEPC(Diethylpyrocarbonate)-treated and sterile filtered water. DEPC inactivates RNase enzymes in water by covalent modifications of the histidine residues. Water, DEPC-Treated is used for the reconstitution of lyophilized products such as oligomers, proteins, and siRNA.


References

1. Huang, Y.H., et al. 1995. Biotechniques. 19: 656-661. PMID: 8777061
2. Mabic, S., et al. 2003. Clin. Chem. Lab. Med. 41: 486-491. PMID: 12747591

Physical State :
Liquid
Solubility :
Soluble in water.
Storage :
Store at room temperature
Melting Point :
0.0° C
Boiling Point :
100° C (lit.)
Density :
1.00 g/mL at 3.98° C (lit.)
Refractive Index :
n20D 1.34 (lit.)
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
nwg
RTECS :
ZC0110000
PubChem CID :
962
Merck Index :
14: 10039
MDL Number :
MFCD00011332
EC Number :
231-791-2
Beilstein Registry :
2050024
SMILES :
O

Download SDS (MSDS)

Certificate of Analysis

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Water, DEPC-Treated  Product Citations

See how others have used Water, DEPC-Treated. Click on the entry to view the PubMed entry .

Citations 1 to 7 of 7 total

PMID: # 25675209  Lee, JH. et al. 2015. Nat Protoc. 10: 442-58.

PMID: # 18056786  Tang, EH. et al. 2008. Physiol. Genomics. 32: 409-418.

PMID: # 17889797  Lu, C. et al. 2007. Methods. 43: 110-117.

PMID: # 17140546  Carra, A. et al. 2007. Anal. Biochem. 360: 318-320.

PMID: # 17545970  Pilcher, KE. et al. 2007. Nat Protoc. 2: 1329-1332.

PMID: # 17571058  Obernosterer, G. et al. 2007. Nat Protoc. 2: 1508-1514.

PMID: # 17406285  Chomczynski, P. et al. 2006. Nat Protoc. 1: 581-585.

Citations 1 to 7 of 7 total

Has this product been autoclaved to remove the DEPC? Thank you in advance for your help, Kate Armfield

Asked by: kfa08
Thank you for your inquiry. This product, DEPC-Treated water, sc-204391, is DEPC-treated, and is guaranteed nuclease-free (tested for both DNase and RNase). If you have any further questions, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: europe@scbt.com or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-02-23
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Rated 5 out of 5 by from Chomczynski Chomczynski, P. et al. (PubMed 17406285) used DEPC-treated water to avoid RNase contamination during reverse transcription of target RNA and PCR assay. -SCBT Publication Review
Date published: 2015-04-04
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