Vimentin Antibody (E-5) recommended for detection of Vimentin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Vimentin Antibody (E-5): sc-373717

Vimentin Antibody (E-5) is rated 4.8 out of 5 by 33.
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Datasheets
    • Vimentin Antibody (E-5) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 419-438 near the C-terminus of Vimentin of human origin
    • recommended for detection of Vimentin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and canine and bovine
    • available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • blocking peptide, sc-373717 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact us to receive a FREE 10 µg sample of Vimentin (E-5): sc-373717.
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Is there data showing reactivity with canine samples?

Asked by: AbPolly
Thank you for your inquiry. We do not have data showing reactivity of this antibody with canine samples, the recommendation for reactivity with canine samples is made based on 100% homology of the epitope of the antibody with the sequence for canine Vimentin.
Answered by: Technical Support 12
Date published: 2017-01-04
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Rated 5 out of 5 by from Works well at 1:50 for immunofluorescence Good staining using 1:50 for immunofluorescence in primary fibroblasts
Date published: 2018-08-10
Rated 4 out of 5 by from Good product It is good antibodies in WB. It works in dilution 1:1000.
Date published: 2018-07-14
Rated 3 out of 5 by from Vimentin Antibody (E-5) Vimentin Antibody (E-5): sc. Near-infrared western blot analysis of LDH-A expression in MEF whole cell lysates. Blocked with 5% NFDM for 1 hour. Detection reagent used: DAM CW800 (Licor).
Date published: 2018-06-28
Rated 4 out of 5 by from きれいにバンドを出すことができました。 Ishikawa細胞 10 μg を泳動しました。非特異的なバンドもでてしまいますが、比較的きれいにバンドを出すことができました。
Date published: 2018-03-16
Rated 5 out of 5 by from antibody The antibody concentration needs to be high to make a positive result.
Date published: 2018-03-07
Rated 5 out of 5 by from NICE i recived it at one month ago,it is very well in WB
Date published: 2018-01-05
Rated 5 out of 5 by from great antibocy It worked well for IF on mouse and rat cardio fibroblasts.
Date published: 2017-12-14
Rated 5 out of 5 by from DOES cross-react with equine cell lines I was kindly provided with an aliquot of this product to test in flow citometry in horse cells. Using human positive controls and 3 different cells (dermal fibroblasts, endothelial and kidney cells), the product crossreacted with equine cells.
Date published: 2017-12-04
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