VCAM-1 Antibody (M/K-2) recommended for detection of VCAM-1 of mouse, rat, human and porcine origin by WB, IP, IF, IHC(P) and FCM

VCAM-1 Antibody (M/K-2): sc-18864

VCAM-1 Antibody (M/K-2) is rated 4.1 out of 5 by 7.
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Datasheets
    • VCAM-1 Antibody (M/K-2) is a rat monoclonal IgG1 provided at 200 µg/ml
    • raised against stromal cells from mouse bone marrow
    • recommended for detection of VCAM-1 of mouse, rat, human and porcine origin by WB, IP, IF, IHC(P) and FCM
    • available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also provided azide-free for blocking, sc-18864 L, 200 µg/0.1 ml
    • Contact us to receive a FREE 10 µg sample of VCAM-1 (M/K-2): sc-18864.
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Can VCAM-1 (M/K-2): sc-18864 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: DefinitelyNotMatt
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, VCAM-1 (M/K-2): sc-18864 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Highly recommended for IHC(P) Produced beautiful cytoplasmic immunoperoxidase staining of formalin fixed, paraffin-embedded human adrenal gland tissue
Date published: 2017-02-08
Rated 4 out of 5 by from Robust band seen in WB using Sol8 whole cell Robust band seen in WB using Sol8 whole cell lysate. -SCBT QC
Date published: 2015-05-05
Rated 4 out of 5 by from Using in WB and IF with excellent results Using in WB and IF with excellent results.
Date published: 2015-04-24
Rated 4 out of 5 by from Publishable data in FCM using human HUVECs Publishable data in FCM using human HUVECs. -SCBT Publication Review
Date published: 2015-04-06
Rated 4 out of 5 by from Publishable data in ICC using human THBMEC Publishable data in ICC using human THBMEC cell lines. -SCBT Publication Review
Date published: 2015-03-23
Rated 4 out of 5 by from Produced wonderful immunoperoxidase cytoplasmic Produced wonderful immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human adrenal gland tissue. -SCBT QC
Date published: 2015-02-13
Rated 4 out of 5 by from Publishable data in WB using human HSE cell lines Publishable data in WB using human HSE cell lines. -SCBT Publication Review
Date published: 2015-02-07
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