Urea Used to denature proteins or in polyacrylamide gels to fractionate low molecular weight DNA or RNA

Urea (CAS 57-13-6)

Urea | CAS 57-13-6 is rated 5.0 out of 5 by 1.
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Synonym: Carbamide
Application: Used to denature proteins or in polyacrylamide gels to fractionate low molecular weight DNA or RNA
CAS Number: 57-13-6
Purity: ≥99%
Molecular Weight: 60.06
Molecular Formula: CH4N2O
* Refer to Certificate of Analysis for lot specific data (including water content).
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Urea can be used as a detergent, similar to guanidine hydrochloride (sc-202637), to denature proteins at high concentrations, or in cell culture to increase the osmolarity. Transformed microorganisms often produce inclusion bodies, which are insoluble, inactive bodies, that form during recombinant protein production. These inclusion bodies can be denatured with urea or guanidine hydrochloride. The compound prefers to interacts with non-polar and aromatic sides, and can be used in polyacrylamide gels containing urea to fractionate low molecular weight DNA or RNA.


References

1. Maniatis, T. and Efstratiadis, A. 1980. Meth. Enzymol. 65: 299-305. PMID: 6154865
2. Marston, F.A. and Hartley, D.L. 1990. Meth. Enzymol. 182: 264-276. PMID: 2179665
3. Green, R.B., et al. 1996. Am. J. Physiol. 271: F1248-F1254. PMID: 8997400
4. Rudolph, R. and Lilie, H. 1996. FASEB J. 10: 49-56. PMID: 8566547
5. Mukhopadhyay, A. 1997. Adv. Biochem. Eng. Biotechnol. 56: 61-109. PMID: 8939059
6. Tsumoto, K., et al. 2010. Curr Pharm Biotechnol. 11: 309-312. PMID: 20210737
7. Mehrnejad, F., et al. 2010. J Comput Aided Mol Des. PMID: 20697778

Usage :
This product is a high quality reagent, but has not been evaluated for use in cell culture. Please employ appropriate contaminant testing and/or sterilization techniques for the intended application.
Appearance :
Powder
Physical State :
Solid
Solubility :
Soluble in water (800 g/l) at 20° C, concentrated hydrochloric acid, 95% alcohol (100 mg/ml), absolute alcohol (50 mg/ml), methanol (166 mg/ml), glycerol (500 mg/ml), benzene, and ethyl acetate (slightly soluble). Insoluble in chloroform, and ether.
Storage :
Store at room temperature
Melting Point :
132-135° C
Boiling Point :
~196.6° C at 760 mmHg
Density :
~1.2 g/cm3
Refractive Index :
n20D 1.40
pK Values :
pKb: 13.90
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
1
RTECS :
YR6250000
PubChem CID :
1176
Merck Index :
14: 9867
MDL Number :
MFCD00008022
EC Number :
200-315-5
Beilstein Registry :
635724
SMILES :
C(=O)(N)N

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Certificate of Analysis

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Urea  Product Citations

See how others have used Urea. Click on the entry to view the PubMed entry .

Citations 1 to 10 of 14 total

PMID: # 18263586  Chung, MC. et al. 2008. J. Biol. Chem. 283: 9531-9542.

PMID: # 17085445  Metz, S. et al. 2007. J. Biol. Chem. 282: 1238-1248.

PMID: # 17363370  Zofall, M. et al. 2007. J. Biol. Chem. 282: 14065-14072.

PMID: # 16849333  Chen, G. et al. 2006. J. Biol. Chem. 281: 27436-27442.

PMID: # 15737990  Yang, F. et al. 2005. J. Biol. Chem. 280: 17154-17162.

PMID: # 15192109  Kao, YY. et al. 2004. J. Biol. Chem. 279: 34440-34448.

PMID: # 12835318  Floyd, DH. et al. 2003. J. Biol. Chem. 278: 35354-35361.

PMID: # 11711540  Liu, HY. et al. 2002. J. Biol. Chem. 277: 2637-2643.

PMID: # 11744737  Chen, S. et al. 2002. J. Biol. Chem. 277: 6037-6043.

PMID: # 10751420  Hutt, D.M. et al. 2000. J. Biol. Chem. 275: 18511-18519.

Citations 1 to 10 of 14 total
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Rated 5 out of 5 by from Chung Chung, et. al. (PubMed ID 18263586) looked to see if addition of urea (mild denaturant), proteases(Npr599 and InhA) to human plasma at 37 degrees Celsius would result in proteolysis of VWF (von Willebrand factor). -SCBT Publication Review
Date published: 2015-07-15
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